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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TH1L Polyclonal antibody

TH1L Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28417-1-AP

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

Validation Data Gallery

Applications:
  • WB analysis using 28417-1-AP

    WB analysis using 28417-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28417-1-AP (TH1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 28417-1-AP

    IF Staining of HeLa using 28417-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28417-1-AP (TH1L antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, MCF-7 cells
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFCD/TH1L Ready-To-Use IHC Kit. NELFCD/TH1L primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28417-1-AP targets TH1L in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TH1L fusion protein Ag29262 相同性解析による交差性が予測される生物種
Full Name TH1-like (Drosophila)
Calculated molecular weight 66 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC014952
Gene symbol TH1L
Gene ID (NCBI) 51497
RRIDAB_2881137
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TH1L antibody 28417-1-APDownload protocol
IF protocol for TH1L antibody 28417-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols