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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TFIP11 Monoclonal antibody

TFIP11 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A1C8

Cat no : 66114-1-Ig

Synonyms

bK445C9.6, STIP, STIP 1, TFIP11, TIP39

Validation Data Gallery

Applications:
  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • WB analysis using 66114-1-Ig

    WB analysis using 66114-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66114-1-Ig

    WB analysis of HeLa using 66114-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 66114-1-Ig

    WB analysis of human testis using 66114-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 66114-1-Ig

    WB analysis of human brain using 66114-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66114-1-Ig (TFIP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66114-1-Ig

    IHC staining of human breast cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66114-1-Ig

    IHC staining of human prostate cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66114-1-Ig

    IHC staining of human prostate cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66114-1-Ig

    IHC staining of human liver cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66114-1-Ig

    IHC staining of human liver cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66114-1-Ig

    IHC staining of human breast cancer using 66114-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66114-1-Ig (TFIP11 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 66114-1-Ig

    IF Staining of HeLa using 66114-1-Ig

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66114-1-Ig(TFIP11 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of HeLa using 66114-1-Ig

    IF Staining of HeLa using 66114-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TFIP11 antibody (66114-1-Ig, Clone: 1A1C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, LNCaP cells, human testis tissue, human brain tissue, MCF-7 cells, HepG2 cells, Jurkat cells, K-562 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66114-1-Ig targets TFIP11 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TFIP11 fusion protein Ag5821 相同性解析による交差性が予測される生物種
Full Name tuftelin interacting protein 11
Calculated molecular weight 97 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC033080
Gene symbol TFIP11
Gene ID (NCBI) 24144
RRIDAB_2881513
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TFIP11, also named as Tuftelin-interacting protein 11 or Septin and tuftelin-interacting protein 1, is a 837 amino acid protein, which contains one G-patch domain and belongs to the TFP11/STIP family. TFIP11 may localizes in the nucleus and cytoplasm. TFIP11 is involved in pre-mRNA splicing, specifically in spliceosome disassembly during late-stage splicing events. TFIP11 may play a role in the differentiation of ameloblasts and odontoblasts or in the forming of the enamel extracellular matrix.

Protocols

Product Specific Protocols
WB protocol for TFIP11 antibody 66114-1-IgDownload protocol
IHC protocol for TFIP11 antibody 66114-1-IgDownload protocol
IF protocol for TFIP11 antibody 66114-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols