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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TET3 Polyclonal antibody

TET3 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22612-1-AP

Synonyms

KIAA0401, tet oncogene family member 3, TET3

Validation Data Gallery

Applications:
  • IHC staining of mouse brain using 22612-1-AP

    IHC staining of mouse brain using 22612-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22612-1-AP (TET3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 22612-1-AP

    IF Staining of U2OS using 22612-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using TET3 antibody (22612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

22612-1-AP targets TET3 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TET3 fusion protein Ag18379 相同性解析による交差性が予測される生物種
Full Name tet oncogene family member 3
Calculated molecular weight 1660 aa, 179 kDa
GenBank accession numberBC022243
Gene symbol TET3
Gene ID (NCBI) 200424
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ten-eleven translocation protein 3 (TET3) belongs to the family of ten-eleven translocations (TETs) which induce DNA demethylation and gene regulation at the epigenetic level by converting 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) (PMID: 31656201). In ovarian cancer, TET3 is reported as an oncogene or tumor suppressor during tumorigenesis and overexpressed in ovarian cancer tissues (PMID: 27141829).

Protocols

Product Specific Protocols
IHC protocol for TET3 antibody 22612-1-APDownload protocol
IF protocol for TET3 antibody 22612-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols