Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 22020-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 22020-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 22020-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22020-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 22020-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 22020-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 22020-1-AP
IP result of anti-TERF2 (IP:22020-1-AP, 4ug; Detection:22020-1-AP 1:500) with K-562 cells lysate 4000ug.
IHC staining of human gliomas using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brain using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 22020-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

TERF2 Polyclonal antibody

TERF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 22020-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human gliomas tissue, rat brain tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IF	See 1 publications below

Product Information

22020-1-AP targets TERF2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16798 Product name: Recombinant human TERF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-251 aa of BC024890 Sequence: MAGGGGSSDGSGRAAGRRASRSSGRARRGRHEPGLGGPAERGAGEARLEEAVNRWVLKFYFHEALRAFRGSRYGDFRQIRDIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLESAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKKHMSKDPTTQKLRNDLLNIIREKNLAHPVIQNFSYETFQQKMLRFLESHLDDAEPYLLTVRLGPSPITMVCP 相同性解析による交差性が予測される生物種
Full Name	telomeric repeat binding factor 2
Calculated molecular weight	56 kDa
Observed molecular weight	65-69 kDa
GenBank accession number	BC024890
Gene Symbol	TERF2
Gene ID (NCBI)	7014
RRID	AB_10973675
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15554
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRF2, also named as TRBF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TRF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TRF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Protocols

Product Specific Protocols
IHC protocol for TERF2 antibody 22020-1-AP	Download protocol
IP protocol for TERF2 antibody 22020-1-AP	Download protocol
WB protocol for TERF2 antibody 22020-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Mol Biol Lett MCCC2 is a novel mediator between mitochondria and telomere and functions as an oncogene in colorectal cancer Authors - Wanjun Liu View Article
	IF	Proc Natl Acad Sci U S A Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta Authors - Talia Hatkevich View Article
human	WB	Oxid Med Cell Longev Inhibition of TRF2 Leads to Ferroptosis, Autophagic Death, and Apoptosis by Causing Telomere Dysfunction Authors - Qiuhui Yang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 22020-1-AP

WB analysis using 22020-1-AP

WB analysis of NIH/3T3 using 22020-1-AP

WB analysis of HeLa using 22020-1-AP

WB analysis of MCF-7 using 22020-1-AP

WB analysis of K-562 using 22020-1-AP

IP experiment of K-562 using 22020-1-AP

IHC staining of human gliomas using 22020-1-AP

IHC staining of human gliomas using 22020-1-AP

IHC staining of rat brain using 22020-1-AP

IHC staining of rat brain using 22020-1-AP

IHC staining of mouse brain using 22020-1-AP

IHC staining of mouse brain using 22020-1-AP

TERF2 Polyclonal antibody

TERF2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MCCC2 is a novel mediator between mitochondria and telomere and functions as an oncogene in colorectal cancer

Gonadal sex and temperature independently influence germ cell differentiation and meiotic progression in Trachemys scripta

Inhibition of TRF2 Leads to Ferroptosis, Autophagic Death, and Apoptosis by Causing Telomere Dysfunction

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