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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TERF2 Polyclonal antibody

TERF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22020-1-AP

Synonyms

Telomeric DNA binding protein, TERF2, TRBF2, TRF2, TTAGGG repeat binding factor 2

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 22020-1-AP

    WB analysis of Jurkat using 22020-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 22020-1-AP

    WB analysis using 22020-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 22020-1-AP

    WB analysis of HeLa using 22020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 22020-1-AP

    WB analysis of MCF-7 using 22020-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 22020-1-AP

    WB analysis of NIH/3T3 using 22020-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 22020-1-AP

    WB analysis of K-562 using 22020-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 22020-1-AP (TERF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 22020-1-AP

    IP experiment of K-562 using 22020-1-AP

    IP result of anti-TERF2 (IP:22020-1-AP, 4ug; Detection:22020-1-AP 1:500) with K-562 cells lysate 4000ug.

  • IHC staining of human gliomas using 22020-1-AP

    IHC staining of human gliomas using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 22020-1-AP

    IHC staining of human gliomas using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 22020-1-AP

    IHC staining of rat brain using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 22020-1-AP

    IHC staining of rat brain using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 22020-1-AP

    IHC staining of mouse brain using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 22020-1-AP

    IHC staining of mouse brain using 22020-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22020-1-AP (TERF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, NIH/3T3 cells, Jurkat cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman gliomas tissue, rat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22020-1-AP is TERF2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TERF2 fusion protein Ag16798 相同性解析による交差性が予測される生物種
Full Name telomeric repeat binding factor 2
Calculated molecular weight 56 kDa
Observed molecular weight 65-69 kDa
GenBank accession numberBC024890
Gene symbol TERF2
Gene ID (NCBI) 7014
RRIDAB_10973675
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRF2, also named as TRBF2 and Telomeric repeat-binding factor 2, is a 542 amino acid protein, which contains 1 HTH myb-type DNA-binding domain and localizes in the Nucleus. TRF2 binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. TRF2 is a component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. TRF2 together with DCLRE1B/Apollo, is required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B), which is needed for telomere replication during fork passage and prevent aberrant telomere topology. TRF2 recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.

Protocols

Product Specific Protocols
WB protocol for TERF2 antibody 22020-1-APDownload protocol
IHC protocol for TERF2 antibody 22020-1-APDownload protocol
IP protocol for TERF2 antibody 22020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Inhibition of TRF2 Leads to Ferroptosis, Autophagic Death, and Apoptosis by Causing Telomere Dysfunction

Authors - Qiuhui Yang
  • KD Validated
View Article
humanWB

Cell Mol Biol Lett

MCCC2 is a novel mediator between mitochondria and telomere and functions as an oncogene in colorectal cancer

Authors - Wanjun Liu
View Article