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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488 Anti-Mouse TER-119 (TER-119)

TER-119 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

TER-119

Cat no : CL488-65149

Synonyms

Ly76, lymphocyte antigen 76, TER 119, Ter119, TER-119

Validation Data Gallery

Applications:
  • FC experiment of mouse bone marrow cells using CL488-65149

    FC experiment of mouse bone marrow cells using CL488-65149

    1X10^6 mouse bone marrow cells were surface co-stained with APC Anti-Mouse CD45 (30-F11) and 0.1 ug CoraLite® Plus 488 Anti-Mouse TER-119 (CL488-65149, Clone: TER-119) or 0.1 ug isotype control. Cells were not fixed.

Tested Applications

Positive FC detected inmouse bone marrow cells
For other applications, we recommend the unconjugated version of this antibody, 65149-1-Ig

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65149 targets TER-119 in FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Immunogen Day-14 fetal liver cells from a C57BL/6 mouse 相同性解析による交差性が予測される生物種
Full Name lymphocyte antigen 76
Gene symbol Ly76
Gene ID (NCBI) 104231
RRIDAB_2883206
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

TER-119 is a mouse erythroid lineage-specific monoclonal antibody that reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colony-forming unit (CFU-E) activities (PMID: 1975515; 10848813). TER-119 recognizes a 52-kDa molecule on erythrocyte membranes (PMID: 10848813). TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself (PMID: 10848813).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TER-119 antibody CL488-65149Download protocol
Standard Protocols
Click here to view our Standard Protocols