CoraLite® Plus 488 Anti-Mouse TCR Beta (H57-597)

TCR Beta Monoclonal Antibody for FC, IF

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

IF, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

H57-597

Cat no : CL488-65106

Synonyms

T cell receptor beta chain, TCR Beta, Tcrb, TCRβ, Tib



Tested Applications

Positive IF detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65106-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:150-1:600
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

CL488-65106 targets TCR Beta in IF, FC applications and shows reactivity with Mouse samples.

Tested Reactivity Mouse
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Immunogen Affinity purified TCR from mouse DO-11.10 cells 相同性解析による交差性が予測される生物種
Full Name T-cell receptor beta chain
Gene symbol Tcrb
Gene ID (NCBI) 21577
RRIDAB_2883180
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Affinity purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

T-cell receptor (TCR) consists of heterodimeric glycoproteins (TCR alpha/beta or gamma/delta) that demonstrate homology with immunoglobulins (PMID: 2144901). TCR beta is a member of the immunoglobulin superfamily and is expressed on alpha/beta TCR-bearing T cells and thymocytes. The TCR heterodimers are noncovalently associated with CD3. The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta (PMID: 1826255). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 TCR Beta antibody CL488-65106Download protocol
Standard Protocols
Click here to view our Standard Protocols