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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated TCF12 Monoclonal antibody

TCF12 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B8D4

Cat no : CL488-67523

Synonyms

bHLHb20, DNA binding protein HTF4, E box binding protein, HEB, HsT17266, HTF4, TCF 12, TCF12, transcription factor 12, Transcription factor HTF 4

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL488-67523

    FC experiment of HepG2 using CL488-67523

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human TCF12 (CL488-67523, Clone:1B8D4) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy TCF12 Ready-To-Use IHC Kit. TCF12 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67523-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67523 targets TCF12 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TCF12 fusion protein Ag6287 相同性解析による交差性が予測される生物種
Full Name transcription factor 12
Calculated molecular weight 73 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC050556
Gene symbol TCF12
Gene ID (NCBI) 6938
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The product of TCF12 gene (TCF12, also called HTF4 or HEB) is a member of helix-loop-helix (HLH) protein family [PMID:22130667]. TCF12 is a transcriptional regulator that involved in the initiation of neuronal differentiation. It 's also known as E proteins, and activates transcription by binding to the E box (5'-CANNTG-3'), because of their direct DNA (E-box) binding ability [PMID: 19204326]. TCF12 was shown to suppress E-cadherin expression during the early stages of renal tubular epithelial cell dedifferentiation [PMID:17202424].

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 TCF12 antibody CL488-67523Download protocol
Standard Protocols
Click here to view our Standard Protocols