Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | mouse testis tissue, human breast cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 78 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
| IP | See 3 publications below |
| ChIP | See 1 publications below |
Product Information
22006-1-AP targets TBP in WB, IHC, IF, IP, ChIP, ELISA applications and shows reactivity with human, mouse, Rat samples.
| Tested Reactivity | human, mouse, Rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBP fusion protein Ag12383 相同性解析による交差性が予測される生物種 |
| Full Name | TATA box binding protein |
| Calculated molecular weight | 338 aa, 38 kDa |
| Observed molecular weight | mouse/rat 33-36 kDa and human 37-43kDa |
| GenBank accession number | BC110341 |
| Gene Symbol | TBP |
| Gene ID (NCBI) | 6908 |
| RRID | AB_10951514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P20226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBP antibody 22006-1-AP | Download protocol |
| IHC protocol for TBP antibody 22006-1-AP | Download protocol |
| IP protocol for TBP antibody 22006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Hepatology Steatotic hepatocytes release mature VLDL via methionine and tyrosine metabolism in a Keap1-Nrf2 dependent manner. | ||
Redox Biol Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke | ||
Cancer Res DNA of neutrophil extracellular traps binds TMCO6 to impair CD8+ T-cell immunity in hepatocellular carcinoma | ||
J Hazard Mater ZnO NPs delay the recovery of psoriasis-like skin lesions through promoting nuclear translocation of p-NFκB p65 and cysteine deficiency in keratinocytes. |