TBP Polyclonal antibody
TBP Polyclonal Antibody for IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 22006-1-AP
Synonyms
"TBP Antibodies" Comparison
View side-by-side comparison of TBP antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IP detected in | COLO 320 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 46 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 22006-1-AP is TBP Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, monkey, mouse, pig, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TBP fusion protein Ag12383 |
| Full Name | TATA box binding protein |
| Calculated molecular weight | 338 aa, 38 kDa |
| Observed molecular weight | mouse/rat 33-36 kDa and human 37-43kDa |
| GenBank accession number | BC110341 |
| Gene symbol | TBP |
| Gene ID (NCBI) | 6908 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TBP antibody 22006-1-AP | Download protocol |
| IHC protocol for TBP antibody 22006-1-AP | Download protocol |
| IP protocol for TBP antibody 22006-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4. | ||
Front Cell Neurosci IRAK-M Deficiency Exacerbates Ischemic Neurovascular Injuries in Experimental Stroke Mice. | ||
Am J Physiol Cell Physiol Expansion of p63-positive CCSP-positive epithelial cells and altered expressions of p63 isoforms in the lung as novel features of aging. | ||
Cell Rep Ets1-Mediated Acetylation of FoxO1 Is Critical for Gluconeogenesis Regulation during Feed-Fast Cycles. | ||
Cell Cycle A novel function of anaphase promoting complex subunit 10 in tumor progression in non-small cell lung cancer. | ||
J Autoimmun Interleukin-1 receptor associated kinase (IRAK)-M -mediated type 2 microglia polarization ameliorates the severity of experimental autoimmune encephalomyelitis (EAE). |
