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TBP 抗体 45 文献

Rabbit Polyclonal| カタログ番号: 22006-1-AP

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, ELISA
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Images See all

TBP Antibody WB COLO 320 cells 22006-1-AP

COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP (TBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

TBP Antibody IP COLO 320 cells 22006-1-AP

IP Result of anti-TBP (IP:22006-1-AP, 4ug; Detection:22006-1-AP 1:500) with COLO 320 cells lysate 400ug.

TBP Antibody IHC human breast cancer tissue 22006-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22006-1-AP (TBP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

種特異性:
human, mouse, monkey, pig, rat
WB ポジティブ:
COLO 320 cells
IP ポジティブ:
COLO 320 cells
IHC ポジティブ:
human breast cancer tissue
推奨希釈倍率:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
TBP fusion protein Ag12383
正式名:
TATA box binding protein
計算分子量:
338 aa, 38 kDa
検出分子量:
mouse/rat 33-36 kDa and human 37-43kDa
GenBank accession number:
BC110341
Gene ID (NCBI):
6908
遺伝子シンボル:
TBP
別名:
GTF2D, GTF2D1, SCA17, TATA binding factor, TATA box binding protein, TATA box factor, TATA sequence binding protein, TBP, TF2D, TFIID

背景説明

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.


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