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Species Reactivity

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  • Featured Product
  • KD/KO Validated

TBL2 Polyclonal antibody

TBL2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12488-1-AP

Synonyms

TBL2, transducin (beta) like 2, Transducin beta like protein 2, WBSCR13, WS betaTRP

Validation Data Gallery

Applications:
  • WB analysis of COLO 320 using 12488-1-AP

    WB analysis of COLO 320 using 12488-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12488-1-AP

    WB analysis of COLO 320 using 12488-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 12488-1-AP

    WB analysis of mouse pancreas using 12488-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 12488-1-AP

    WB analysis of human placenta using 12488-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12488-1-AP

    WB analysis of Jurkat using 12488-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12488-1-AP

    WB analysis of HeLa using 12488-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12488-1-AP

    WB analysis of COLO 320 using 12488-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of COLO 320 using 12488-1-AP

    WB analysis of COLO 320 using 12488-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse placenta using 12488-1-AP

    WB analysis of mouse placenta using 12488-1-AP

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12488-1-AP (TBL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of COLO 320 using 12488-1-AP

    IP experiment of COLO 320 using 12488-1-AP

    IP result of anti-TBL2 (IP:12488-1-AP, 4ug; Detection:12488-1-AP 1:800) with COLO 320 cells lysate 2400ug.

  • IHC staining of human pancreas cancer using 12488-1-AP

    IHC staining of human pancreas cancer using 12488-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 12488-1-AP

    IHC staining of human heart using 12488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 12488-1-AP

    IHC staining of human heart using 12488-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human pancreas using 12488-1-AP

    IHC staining of human pancreas using 12488-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human pancreas using 12488-1-AP

    IHC staining of human pancreas using 12488-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 12488-1-AP (TBL2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 12488-1-AP

    IF Staining of HepG2 using 12488-1-AP

    Immunofluorescent analysis of () fixed HepG2 cells using 12488-1-AP (TBL2 antibody) at dilution of 1:25.

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, human placenta tissue, Jurkat cells, mouse pancreas tissue, mouse placenta tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman pancreas cancer tissue, human heart tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12488-1-AP targets TBL2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBL2 fusion protein Ag3200 相同性解析による交差性が予測される生物種
Full Name transducin (beta)-like 2
Calculated molecular weight 446 aa, 50 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC012938
Gene symbol TBL2
Gene ID (NCBI) 26608
RRIDAB_2199923
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TBL2 belongs to a member of the beta-transducin protein family. This gene is commonly deleted in Williams-Beuren syndrome, a developmental disorder with multi-system manifestations caused by haploinsufficiency for contiguous genes deleted in chromosome region 7q11.23. TBL2 encodes a protein with four putative WD40 repeats, which may be involved in some intracellular signaling pathway.

Protocols

Product Specific Protocols
WB protocol for TBL2 antibody 12488-1-APDownload protocol
IHC protocol for TBL2 antibody 12488-1-APDownload protocol
IF protocol for TBL2 antibody 12488-1-APDownload protocol
IP protocol for TBL2 antibody 12488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

PLoS One

TBL2 Is a Novel PERK-Binding Protein that Modulates Stress-Signaling and Cell Survival during Endoplasmic Reticulum Stress.

Authors - Yoshinori Tsukumo
  • KD Validated
View Article
WB

J Cell Biochem

TBL2 Associates With ATF4 mRNA Via Its WD40 Domain and Regulates Its Translation During ER Stress.

Authors - Yoshinori Tsukumo
View Article
humanWB, IF

Biochem Biophys Res Commun

The endoplasmic reticulum-localized protein TBL2 interacts with the 60S ribosomal subunit.

Authors - Yoshinori Tsukumo
View Article
humanWB,IHC

Ann Surg Oncol

Transducin Beta-Like 2 is a Potential Driver Gene that Adapts to Endoplasmic Reticulum Stress to Promote Tumor Growth of Lung Adenocarcinoma

Authors - Keisuke Kosai
  • KD Validated
View Article