Validation Data Gallery
Tested Applications
| Positive WB detected in | HT-1080 cells, HCT 116 cells, HeLa cells, HepG2 cells, U2OS cells |
| Positive IHC detected in | human stomach cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 78 publications below |
| IHC | See 3 publications below |
| IP | See 2 publications below |
Product Information
28397-1-AP targets TBK1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, bovine, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28894 Product name: Recombinant human TBK1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 316-576 aa of BC034950 Sequence: LQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLA 相同性解析による交差性が予測される生物種 |
| Full Name | TANK-binding kinase 1 |
| Observed molecular weight | 84 kDa |
| GenBank accession number | BC034950 |
| Gene Symbol | TBK1 |
| Gene ID (NCBI) | 29110 |
| RRID | AB_2881132 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UHD2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TBK1, also named as tumor necrosis factor (TNF) receptor-associated factor NF-kB activator (TANK)-binding kinase 1 (TBK1), NF-kB-activating kinase (NAK), T2K, is a multimeric kinase that modulates inflammation and autophagy. It is a ubiquitously expressed serine-threonine kinase belonging to the 'noncanonical IkB kinases' (IKKs) recognized for its critical role in regulating type I IFN production (PMID: 27211305). And TBK1 is an important player in yet another critical cellular function, autophagy. This antibody is specific for TBK1.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TBK1 antibody 28397-1-AP | Download protocol |
| IHC protocol for TBK1 antibody 28397-1-AP | Download protocol |
| WB protocol for TBK1 antibody 28397-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation | ||
Mol Ther STING/ACSL4 axis-dependent ferroptosis and inflammation promote hypertension-associated chronic kidney disease | ||
Protein Cell Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response. | ||
J Anim Sci Biotechnol β-Hydroxybutyrate-induced mitochondrial DNA (mtDNA) release mediated innate inflammatory response in bovine mammary epithelial cells by inhibiting autophagy | ||
PLoS Pathog SARS-CoV-2 spike-induced syncytia are senescent and contribute to exacerbated heart failure | ||
PLoS Pathog TRIM32 inhibits Venezuelan equine encephalitis virus infection by targeting a late step in viral entry |
