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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Spag8 Polyclonal antibody

Spag8 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30287-1-AP

Synonyms

MH SPAG8, Spag8, sperm associated antigen 8

Validation Data Gallery

Applications:
  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis using 30287-1-AP

    WB analysis using 30287-1-AP

    rat testis tissue lysates were subjected to SDS PAGE followed by western blot with 30287-1-AP (Spag8 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 30287-1-AP

    IP experiment of mouse testis using 30287-1-AP

    IP result of anti-Spag8 (IP:30287-1-AP, 4ug; Detection:30287-1-AP 1:3000) with mouse testis tissue lysate 1120 ug.

  • IHC staining of mouse testis using 30287-1-AP

    IHC staining of mouse testis using 30287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 30287-1-AP

    IHC staining of mouse testis using 30287-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30287-1-AP (Spag8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30287-1-AP targets Spag8 in WB, IP, IHC, ELISA applications and shows reactivity with rat, mouse samples.

Tested Reactivity rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Spag8 fusion protein Ag31603 相同性解析による交差性が予測される生物種
Full Name sperm associated antigen 8
Observed molecular weight60-70 kDa
GenBank accession numberBC150868
Gene symbol Spag8
Gene ID (NCBI) 433700
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Spag8 antibody 30287-1-APDownload protocol
IHC protocol for Spag8 antibody 30287-1-APDownload protocol
IP protocol for Spag8 antibody 30287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols