Synaptophysin Polyclonal antibody

Synaptophysin Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17785-1-AP

Synonyms

SYN, synaptophysin, SYP



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse pancreas tissue, mouse brain tissue, human brain tissue, ganglioma tissue, human pancreas tissue, neuroblastoma tissue, human pituitary adenoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse pancreas tissue, PC-12 cells, human lung cancer tissue, mouse brain tissue, human gliomas tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17785-1-AP targets Synaptophysin in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse, hamster, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Synaptophysin fusion protein Ag11781 相同性解析による交差性が予測される生物種
Full Name synaptophysin
Calculated molecular weight 313 aa, 34 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC064550
Gene symbol SYP
Gene ID (NCBI) 6855
RRIDAB_2271365
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptophysin is a useful marker for identification of neuroendocrine cells and neoplasms. (PMID: 3010302; 21658579)

Protocols

Product Specific Protocols
WB protocol for Synaptophysin antibody 17785-1-APDownload protocol
IHC protocol for Synaptophysin antibody 17785-1-APDownload protocol
IF protocol for Synaptophysin antibody 17785-1-APDownload protocol
IP protocol for Synaptophysin antibody 17785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
mouse, ratWB,IF

Nat Genet

Identification of TMEM230 mutations in familial Parkinson's disease.

Authors - Han-Xiang Deng
mouseWB,IF

Nat Neurosci

Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage

Authors - Yao Wei
mouseIF

Mol Cell

Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.

Authors - Zhengxin Ying
mouseWB

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
mouseIF

Neuron

Loss of PYCR2 Causes Neurodegeneration by Increasing Cerebral Glycine Levels via SHMT2.

Authors - Nathalie Escande-Beillard

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandra (Verified Customer) (06-11-2023)

Hoechst-blue Green-Tubulin III (neuronal marker) Orange (Synaptophysin)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: P19 derived neurons
Synaptophysin Antibody Immunofluorescence validation (1:300 dilution) in P19 derived neurons (Cat no:17785-1-AP)
FH

Azita (Verified Customer) (05-31-2021)

Human primary cortical neurons was labelled with 1/1000 of the Synaptophysin Polyclonal antibody. The labelling was strong and very good. Blue (Bisbenzimide) Green (Synaptophysin)

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Human primary cortical neurons
Synaptophysin Antibody Immunohistochemistry validation (1/1000 dilution) in Human primary cortical neurons (Cat no:17785-1-AP)