Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive IF detected in | Human dorsal cerebral organoid, mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
25265-1-AP targets Nesprin 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | spectrin repeat containing, nuclear envelope 2 |
| Calculated molecular weight | 799 kDa |
| Observed molecular weight | 50-60 kDa |
| GenBank accession number | NM_015180 |
| Gene Symbol | Nesprin 2 |
| Gene ID (NCBI) | 23224 |
| RRID | AB_2879995 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WXH0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Nesprin-2 is a member of actin-binding proteins that localize to the outer nuclear membrane. As a nuclear envelope component it provides a link between cytoskeletal components of the cytoplasm and the nucleoplasm. Nesprin genes generate, through alternative splicing and alternative promoter and/or terminator usage, a multitude of differentially expressed isoforms, differing in domain architecture and length. The largest protein isoform, known as NUANCE or GIANT, has a predicted MW around 796 kDa. Other isoforms range from 30 kDa to 119 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| WB protocol for Nesprin 2 antibody 25265-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
