Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, HEK-293 cells, HeLa cells, HepG2 cells, mouse small intestine tissue, C6 cells, NIH/3T3 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 18 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10574-1-AP targets SUV39H1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0854 Product name: Recombinant human SUV39H1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-271 aa of BC006238 Sequence: GCSVCCKSSWNQLQDLCRLAKLSCPALGISKRNLYDFEVEYLCDYKKIREQEYYLVKWRGYPDSESTWEPRQNLKCVRILKQFHKDLERELLRRHHRSKTPRHLDPSLANYLVQKAKQRRALRRWEQELNAKRSHLGRITVENEVDLDGPPRAFVYINEYRVGEGITLNQVAVGCECQDCLWAPTGGCCPGASLHKFAYNDQGQVRLRAGLPIYECNSRCRCGYDCPNRVVQKGIRYDLCIFRTDDGRGWGVRTLEKIRKNSFVM 相同性解析による交差性が予測される生物種 |
| Full Name | suppressor of variegation 3-9 homolog 1 (Drosophila) |
| Calculated molecular weight | 48 kDa |
| Observed molecular weight | 48-50 kDa |
| GenBank accession number | BC006238 |
| Gene Symbol | SUV39H1 |
| Gene ID (NCBI) | 6839 |
| RRID | AB_2196710 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43463 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Histone-lysine N-methyltransferase (SUV39H1), a nuclear protein, moves to the centromeres during mitosis and specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. It plays a vital role in heterochromatin organization, chromosome segregation, and mitotic progression. SUV39H1 has 2 isoforms with molecular weights of 48 and 49 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| WB protocol for SUV39H1 antibody 10574-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication | ||
Nat Commun The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.
| ||
J Med Chem Discovery of a Novel Small-Molecule Inhibitor Disrupting TRBP-Dicer Interaction against Hepatocellular Carcinoma via the Modulation of microRNA Biogenesis. | ||
Cell Death Dis Dicer promotes tumorigenesis by translocating to nucleus to promote SFRP1 promoter methylation in cholangiocarcinoma cells. | ||
Oncotarget Downregulation of HP1α suppresses proliferation of cholangiocarcinoma by restoring SFRP1 expression. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |
