Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 3 publications below |
Product Information
11675-1-AP targets SUGT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2275 Product name: Recombinant human SUGT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-333 aa of BC000911 Sequence: MAAAAAGTATSQRFFQSFSDALIDEDPQAALEELTKALEQKPDDAQYYCQRAYCHILLGNYCVAVADAKKSLELNPNNSTAMLRKGICEYHEKNYAAALETFTEGQKLDSADANFSVWIKRCQEAQNGSESEVWTHQSKIKYDWYQTESQVVITLMIKNVQKNDVNVEFSEKELSALVKLPSGEDYNLKLELLHPIIPEQSTFKVLSTKIEIKLKKPEAVRWEKLEGQGDVPTPKQFVADVKNLYPSSSPYTRNWDKLVGEIKEEEKNEKLEGDAALNRLFQQIYSDGSDEVKRAMNKSFMESGGTVLSTNWSDVGKRKVEINPPDDMEWKKY 相同性解析による交差性が予測される生物種 |
| Full Name | SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae) |
| Calculated molecular weight | 41 kDa |
| Observed molecular weight | 35-41 kDa |
| GenBank accession number | BC000911 |
| Gene Symbol | SUGT1 |
| Gene ID (NCBI) | 10910 |
| RRID | AB_2197436 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2Z0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SUGT1 antibody 11675-1-AP | Download protocol |
| IHC protocol for SUGT1 antibody 11675-1-AP | Download protocol |
| IP protocol for SUGT1 antibody 11675-1-AP | Download protocol |
| WB protocol for SUGT1 antibody 11675-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol HSF1 Protects Sepsis-Induced Acute Lung Injury by Inhibiting NLRP3 Inflammasome Activation. | ||
PLoS One Calcyclin Binding Protein/Siah-1 Interacting Protein Is a Hsp90 Binding Chaperone. | ||
Int J Oncol Overexpression of SUGT1 in human colorectal cancer and its clinicopathological significance. | ||
JCI Insight Echinatin effectively protects against NLRP3 inflammasome-driven diseases by targeting HSP90. | ||
Eur J Med Res SUGT1 is a prognostic biomarker and is associated with immune infiltrates in ovarian cancer |