Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse lung tissue, DU 145 cells, human placenta tissue, human skeletal muscle tissue, Jurkat cells, L02 cells, mouse placenta tissue, rat lung tissue |
| Positive IP detected in | DU 145 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 16 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
Product Information
13301-1-AP targets Syntaxin 11 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4097 Product name: Recombinant human STX11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC033519 Sequence: MKDRLAELLDLSKQYDQQFPDGDDEFDSPHEDIVFETDHILESLYRDIRDIQDENQLLVADVKRLGKQNARFLTSMRRLSSIKRDTNSIAKAIKARGEVIHCKLRAMKELSEAAEAQHGPHSAVARISRAQYNALTLTFQRAMHDYNQAEMKQRDNCKIRIQRQLEIMGKEVSGDQIEDMFEQGKWDVFSENLLADVKGARAALNEIESRHRELLRLESRIRDVHELFLQMAVLVEKQADTLNVIELNVQKTVDYTGQAKAQVRKAVQYEEKNPCRTLCCFCCPCLK 相同性解析による交差性が予測される生物種 |
| Full Name | syntaxin 11 |
| Calculated molecular weight | 287 aa, 33 kDa |
| Observed molecular weight | 33-35 kDa |
| GenBank accession number | BC033519 |
| Gene Symbol | Syntaxin 11 |
| Gene ID (NCBI) | 8676 |
| RRID | AB_2198214 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75558 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Syntaxin 11 (STX11) belongs to the syntaxin family. It interacts with the SNARE proteins SNAP-23 and VAMP. STX11 behaves as an integral membrane protein despite the lack of a traditional transmembrane domain. The detection of STX11 in human cytotoxic lymphocytes suggests that this protein could play a role in the process leading to degranulation of secretory lysosomes (PMID:17525286).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| IP protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| WB protocol for Syntaxin 11 antibody 13301-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion
| ||
J Exp Med Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. | ||
Proc Natl Acad Sci U S A Crucial role of the hydrophobic pocket region of Munc18 protein in mast cell degranulation. | ||
J Cell Sci Chaperoning of closed syntaxin-3 through Lys46 and Glu59 in domain-1 of Munc18 is indispensable for mast cell exocytosis. | ||
Front Immunol Bi-Allelic Mutations in STXBP2 Reveal a Complementary Role for STXBP1 in Cytotoxic Lymphocyte Killing. |
