Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human ovary tumor tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 7 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| IP | See 2 publications below |
Product Information
18277-1-AP targets STRAP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13085 Product name: Recombinant human STRAP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-350 aa of BC000162 Sequence: MAMRQTPLTCSGHTRPVVDLAFSGITPYGYFLISACKDGKPMLRQGDTGDWIGTFLGHKGAVWGATLNKDATKAATAAADFTAKVWDAVSGDELMTLAHKHIVKTVDFTQDSNYLLTGGQDKLLRIYDLNKPEAEPKEISGHTSGIKKALWCSEDKQILSADDKTVRLWDHATMTEVKSLNFNMSVSSMEYIPEGEILVITYGRSIAFHSAVSLDPIKSFEAPATINSASLHPEKEFLVAGGEDFKLYKYDYNSGEELESYKGHFGPIHCVRFSPDGELYASGSEDGTLRLWQTVVGKTYGLWKCVLPEEDSGELAKPKIGFPETTEEELEEIASENSDCIFPSAPDVKA 相同性解析による交差性が予測される生物種 |
| Full Name | serine/threonine kinase receptor associated protein |
| Calculated molecular weight | 38 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC000162 |
| Gene Symbol | STRAP |
| Gene ID (NCBI) | 11171 |
| RRID | AB_2255489 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y3F4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STRAP (Serine-threonine kinase receptor-associated protein), also known as MAWD, is a ubiquitous WD40 domain protein, common function of which is to provide a suitable scaffold for coordinating multiprotein complex assemblies and thus, regulate a variety of cellular processes like signal transduction, transcriptional regulation, programmed cell death and so on. STRAP plays a role in the cellular distribution of the SMN complex, which is essential for spliceosomal snRNP assembly in the cytoplasm. STRAP inhibits transforming growth factor-beta (TGF-beta) signaling and enhances tumorigenicity via TGF-beta-dependent and -independent mechanisms. STRAP is up-regulated in several cancers and functions as an oncogene. STRAP imparts oncogenic characteristics to cells by promoting ERK and pRb phosphorylation. Moreover, STRAP regulates c-Jun stability by decreasing the ubiquitylation and proteosomal degradation of c-Jun.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STRAP antibody 18277-1-AP | Download protocol |
| IHC protocol for STRAP antibody 18277-1-AP | Download protocol |
| IP protocol for STRAP antibody 18277-1-AP | Download protocol |
| WB protocol for STRAP antibody 18277-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Comprehensive analysis of the BC200 ribonucleoprotein reveals a reciprocal regulatory function with CSDE1/UNR.
| ||
EMBO J The p53-inducible long noncoding RNA TRINGS protects cancer cells from necrosis under glucose starvation.
| ||
Cancers (Basel) Serine-Threonine Kinase Receptor-Associated Protein (STRAP) Knockout Decreases the Malignant Phenotype in Neuroblastoma Cell Lines.
| ||
Front Oncol STRAP as a New Therapeutic Target for Poor Prognosis of Pancreatic Ductal Adenocarcinoma Patients Mainly Caused by TP53 Mutation.
| ||
J Biol Chem The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells. | ||
