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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated STRA8 Monoclonal antibody

STRA8 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rabbit, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D7E3

Cat no : CL488-68071

Synonyms

STRA8

Validation Data Gallery

Applications:
  • IF Staining of NCCIT using CL488-68071

    IF Staining of NCCIT using CL488-68071

    Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using CoraLite® Plus 488 STRA8 antibody (CL488-68071, Clone: 1D7E3 ) at dilution of 1:200.

Tested Applications

Positive IF detected inNCCIT cells
For other applications, we recommend the unconjugated version of this antibody, 68071-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-68071 targets STRA8 in IF applications and shows reactivity with Human, rabbit, mouse samples.

Tested Reactivity Human, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen STRA8 fusion protein Ag29680 相同性解析による交差性が予測される生物種
Full Name stimulated by retinoic acid gene 8 homolog (mouse)
Calculated molecular weight 37 kDa
Observed molecular weight 43-60 kda
GenBank accession numberNM_182489
Gene symbol STRA8
Gene ID (NCBI) 346673
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stimulated by retinoic acid 8 (Stra8), one of genes induced by retinoic acid (RA), is required for the meiotic initiation of male spermatogenesis. Stimulated by Retinoic Acid Gene 8 (STRA8) protein has been proposed as the molecular effector of Retinoic Acid (RA) involved in meiosis-inductive activity. Stra8 is a 45-kDa vertebrate-specific gene, without significant homologous proteins, expressed in the cytoplasm and nuclei of germ cells between mitosis and meiosis and presents exclusively in the embryonic and postnatal gonads of mammals. (PMID: 30106128, PMID: 31253359)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 STRA8 antibody CL488-68071Download protocol
Standard Protocols
Click here to view our Standard Protocols