Validation Data Gallery

Filter: AllWBIHC

WB analysis of HepG2 using 17186-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 17186-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17186-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 17186-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17186-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for ELISA

Cat No. 17186-1-AP

Publications(17)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (17)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

17186-1-AP targets STEAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10970 Product name: Recombinant human STEAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC042150 Sequence: MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAM 相同性解析による交差性が予測される生物種
Full Name	STEAP family member 3
Calculated molecular weight	488 aa, 55 kDa
GenBank accession number	BC042150
Gene Symbol	STEAP3
Gene ID (NCBI)	55240
RRID	AB_2197841
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q658P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for STEAP3 antibody 17186-1-AP	Download protocol
WB protocol for STEAP3 antibody 17186-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Authors - Steinberg Florian F View Article
human	WB	Nat Commun Regulation of the endosomal SNX27-retromer by OTULIN. Authors - Aurelia Stangl View Article
mouse	WB	J Exp Med Ferrous iron-activatable drug conjugate achieves potent MAPK blockade in KRAS-driven tumors. Authors - Honglin Jiang View Article
mouse	WB	Int J Biol Macromol Exosomes from tannic acid-stimulated macrophages accelerate wound healing through miR-221-3p mediated fibroblasts migration by targeting CDKN1b Authors - Xu Song View Article
human	WB	J Nucl Med Ferronostics: Measuring Tumoral Ferrous Iron with PET to Predict Sensitivity to Iron-Targeted Cancer Therapies. Authors - Ning Zhao View Article
mouse	WB	Blood Adv Differences in Steap3 expression are a mechanism of genetic variation of RBC storage and oxidative damage in mice. Authors - Heather L Howie View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 17186-1-AP

WB analysis of C6 using 17186-1-AP

WB analysis of HepG2 using 17186-1-AP

IHC staining of human liver using 17186-1-AP

IHC staining of human liver using 17186-1-AP

STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.

Regulation of the endosomal SNX27-retromer by OTULIN.

Ferrous iron-activatable drug conjugate achieves potent MAPK blockade in KRAS-driven tumors.

Exosomes from tannic acid-stimulated macrophages accelerate wound healing through miR-221-3p mediated fibroblasts migration by targeting CDKN1b

Ferronostics: Measuring Tumoral Ferrous Iron with PET to Predict Sensitivity to Iron-Targeted Cancer Therapies.

Differences in Steap3 expression are a mechanism of genetic variation of RBC storage and oxidative damage in mice.