Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, SW480 cells, U-251 cells |
| Positive IP detected in | SW480 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 4 publications below |
| WB | See 13 publications below |
| IHC | See 7 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
20621-1-AP targets STC1 in WB, IHC, IF, IP, ELISA, Cell treatment applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14697 Product name: Recombinant human STC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 98-247 aa of BC029044 Sequence: CIANGVTSKVFLAIRRCSTFQRMIAEVQEECYSKLNVCSIAKRNPEAITEVVQLPNHFSNRYYNRLVRSLLECDEDTVSTIRDSLMEKIGPNMASLFHILQTDHCAQTHPRADFNRRRTNEPQKLKVLLRNLRGEEDSPSHIKRTSHESA 相同性解析による交差性が予測される生物種 |
| Full Name | stanniocalcin 1 |
| Calculated molecular weight | 247 aa, 28 kDa |
| Observed molecular weight | 25-28 kDa |
| GenBank accession number | BC029044 |
| Gene Symbol | STC1 |
| Gene ID (NCBI) | 6781 |
| RRID | AB_10694290 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P52823 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
STC1, also named as stanniocalcin 1, a 56kDa homodimeric glycoprotein hormone, is highly expressed in a variety of cancers such as breast, ovarian, and colorectal cancers (PMID: 27459971). This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. STC1 is implicated in several pathologies including retinal degeneration, cerebral ischemia, angiogenesis and inflammation (PMID: 25391215). The calculated molecular weight of STC1 is usually approximately 28 kda.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for STC1 antibody 20621-1-AP | Download protocol |
| IP protocol for STC1 antibody 20621-1-AP | Download protocol |
| WB protocol for STC1 antibody 20621-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Lactylation-driven NSUN2-mediated RNA m5C modification promotes perineural invasion in pancreatic cancer. | ||
J Exp Clin Cancer Res Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer.
| ||
Cancer Cell Int Gastric cancer cell-originated small extracellular vesicle induces metabolic reprogramming of BM-MSCs through ERK-PPARγ-CPT1A signaling to potentiate lymphatic metastasis | ||
Front Immunol Identification of prognosis and therapy related intratumoral microbiome and immune signatures in gastric cancer | ||
Life Sci STC-1 improves scar remodeling and is associated with PI3K/AKT signaling and immune modulation. |