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STAT3 抗体 23 文献

Mouse Monoclonal| カタログ番号: 60199-1-Ig |クローン番号: 3G2D12

注目製品 KD/KO 検証済

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, ELISA
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Images See all

STAT3 Antibody WB HeLa cells 60199-1-Ig

WB result of STAT3 antibody (60199-1-Ig, 1:3000) with si-Control and si-STAT3 transfected HeLa cells.

STAT3 Antibody FC HepG2 cells 60199-1-Ig

1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human STAT3 (60199-1-Ig, Clone:3G2D12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

STAT3 Antibody WB HeLa cells 60199-1-Ig

Western blot analysis of STAT3 in various cell lines using Proteintech antibody 60199-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours.

STAT3 Antibody IP HeLa cells 60199-1-Ig

IP Result of anti-STAT3 (IP:60199-1-Ig, 5ug; Detection:60199-1-Ig 1:2000) with HeLa cells lysate 1400ug.

STAT3 Antibody IHC human cervical cancer tissue 60199-1-Ig

Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60199-1-Ig (STAT3 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

STAT3 Antibody IF HepG2 cells 60199-1-Ig

Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60199-1-Ig(STAT3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

種特異性:
human, mouse, rat
WB ポジティブ:
HeLa cells, HeLa
IP ポジティブ:
HeLa cells
IHC ポジティブ:
human cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue
IF ポジティブ:
HepG2 cells
FC ポジティブ:
HepG2 cells
推奨希釈倍率:
WB : 1:2000-1:20000
IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Mouse
精製方法:
Protein A purification
アイソタイプ:
IgG2a
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
STAT3 fusion protein Ag0360
正式名:
signal transducer and activator of transcription 3 (acute-phase response factor)
計算分子量:
770 aa, 88 kDa
検出分子量:
88 kDa
GenBank accession number:
BC000627
Gene ID (NCBI):
6774
遺伝子シンボル:
STAT3
別名:
Acute phase response factor, APRF, HIES, STAT3

背景説明

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.


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