Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 10583-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 10583-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10583-1-AP
WB result of TRAPA/SSR1 antibody (10583-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAPA/SSR1 transfected HepG2 cells.
WB analysis of human liver using 10583-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 10583-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis using 10583-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 10583-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10583-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 10583-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 10583-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 10583-1-AP (TRAPA/SSR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HEK-293 using 10583-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using TRAPA/SSR1 antibody (10583-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

TRAPA/SSR1 Polyclonal antibody

TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 10583-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, monkey and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SSR1, TRAP alpha, PSEC0262, SSR alpha, SSR-alpha

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells
Positive IHC detected in	human ovary tumor tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IHC	See 1 publications below
IF	See 3 publications below

Product Information

10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity	human, mouse, monkey
Cited Reactivity	human, rat, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0878 Product name: Recombinant human TRAPA, SSR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-286 aa of BC007710 Sequence: MRLLPRLLLLLLLVFPATVLFRGGPRGLLAVAQDLTEDEETVEDSIIEDEDDEAEVEEDEPTDLVEDKEEEDVSGEPEASPSADTTILFVKGEDFPANNIVKFLVGFTNKGTEDFIVESLDASFRYPQDYQFYIQNFTALPLNTVVPPQRQATFEYSFIPAEPMGGRPFGLVINLNYKDLNGNVFQDAVFNQTVTVIEREDGLDGETIFMYMFLAGLGLLVIVGLHQLLESRKRKRPIQKVEMGTSSQNDVDMSWIPQETLNQINKASPRRLPRKRAQKRSVGSDE 相同性解析による交差性が予測される生物種
Full Name	signal sequence receptor, alpha
Calculated molecular weight	34 kDa
Observed molecular weight	34 kDa
GenBank accession number	BC007710
Gene Symbol	TRAPA
Gene ID (NCBI)	6745
RRID	AB_2195616
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P43307
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).

Protocols

Product Specific Protocols
IF protocol for TRAPA/SSR1 antibody 10583-1-AP	Download protocol
IHC protocol for TRAPA/SSR1 antibody 10583-1-AP	Download protocol
WB protocol for TRAPA/SSR1 antibody 10583-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng View Article
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
human	WB	Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. Authors - Chatchai Phoomak View Article
human	IF	Biosens Bioelectron Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. Authors - Mao Chen View Article
human	WB	FASEB J GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin. Authors - S Orvokki Mattila View Article
rat	WB,IF	Exp Cell Res Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers. Authors - Kaakinen Mika M View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10583-1-AP

WB analysis using 10583-1-AP

WB analysis of HepG2 using 10583-1-AP

WB analysis of human liver using 10583-1-AP

WB analysis of mouse liver using 10583-1-AP

WB analysis using 10583-1-AP

IHC staining of human ovary tumor using 10583-1-AP

IHC staining of human ovary tumor using 10583-1-AP

IHC staining of human lung using 10583-1-AP

IHC staining of human lung using 10583-1-AP

IF Staining of HEK-293 using 10583-1-AP

TRAPA/SSR1 Polyclonal antibody

TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

ER proteins decipher the tubulin code to regulate organelle distribution

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.

GPR37 is processed in the N-terminal ectodomain by ADAM10 and furin.

Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers.