Spermidine synthase Polyclonal antibody
Spermidine synthase Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19858-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, LNCaP cells, HepG2 cells, PC-3 cells, mouse liver tissue, rat liver tissue, RAW 264.7 cells, mouse brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 11 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
19858-1-AP targets Spermidine synthase in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Spermidine synthase fusion protein Ag13936 相同性解析による交差性が予測される生物種 |
| Full Name | spermidine synthase |
| Calculated molecular weight | 302 aa, 34 kDa |
| Observed molecular weight | 34 kDa |
| GenBank accession number | BC000309 |
| Gene symbol | SRM |
| Gene ID (NCBI) | 6723 |
| RRID | AB_10665555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Spermidine synthase is an enzyme that catalyzes the transfer of the propylamine group from S-adenosylmethioninamine to putrescine in the biosynthesis of spermidine. The polyamines putrescine, spermine, and spermidine are ubiquitous polycationic mediators of cell growth and differentiation. Spermidine synthases catalyze the production of the linear triamine, spermidine, from putrescine. They utilize decarboxylated S-adenosylmethionine (dc-SAM), a universal cofactor of aminopropyltransferases, as a donor of the aminopropyl moiety (PMID:31134111).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IHC protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IF protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| IP protocol for Spermidine synthase antibody 19858-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage ABHD5 promotes colorectal cancer growth by suppressing spermidine production by SRM. | ||
Cancer Sci The miR-1908/SRM regulatory axis contributes to extracellular vesicle secretion in prostate cancer. | ||
J Proteomics Proteomic analysis revealed different responses to hypergravity of soleus and extensor digitorum longus muscles in mice. | ||
Am J Physiol Cell Physiol The Regulation of Polyamine Pathway Proteins in Models of Skeletal Muscle Hypertrophy and Atrophy - a potential role for mTORC1. | ||
Biol Reprod Progesterone and interferon tau regulate expression of polyamine enzymes during the ovine peri-implantation period. |
