Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 14508-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14508-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 14508-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14508-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 14508-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 14508-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 14508-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 14508-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 14508-1-AP (SREBF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14508-1-AP
IP result of anti-SREBF2 (IP:14508-1-AP, 3ug; Detection:14508-1-AP 1:500) with HeLa cells lysate 3000ug.
IHC staining of mouse brain using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 14508-1-AP (SREBF2 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver cancer using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 14508-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14508-1-AP (SREBF2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SREBF2 Polyclonal antibody

SREBF2 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14508-1-AP

Publications(38)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, chIP, ELISA

Synonyms

bHLHd2, SREBF2, SREBP 2, SREBP2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (38)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse kidney tissue, HepG2 cells, human kidney tissue, HEK-293 cells, rat kidney tissue, HeLa cells, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human kidney tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 36 publications below
IHC	See 3 publications below
IF	See 1 publications below
ChIP	See 1 publications below

Product Information

14508-1-AP targets SREBF2 in WB, IHC, IF, IP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, chicken, hamster
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5967 Product name: Recombinant human SREBF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 795-1141 aa of BC056158 Sequence: PIAQVHQAFCKNLLERAIESLVKPQAKKKAGDQEEESCEFSSALEYLKLLHSFVDSVGVMSPPLSRSSVLKSALGPDIICRWWTSAITVAISWLQGDDAAVRSHFTKVERIPKALEVTESPLVKAIFHACRAMHASLPGKADGQQSSFCHCERASGHLWSSLNVSGATSDPALNHVVQLLTCDLLLSLRTALWQKQASASQAVGETYHASGAELAGFQRDLGSLRRLAHSFRPAYRKVFLHEATVRLMAGASPTRTHQLLEHSLRRRTTQSTKHGEVDAWPGQRERATAILLACRHLPLSFLSSPGQRAVLLAEAARTLEKVGDRRSCNDCQQMIVKLGGGTAIAAS 相同性解析による交差性が予測される生物種
Full Name	sterol regulatory element binding transcription factor 2
Calculated molecular weight	124 kDa
Observed molecular weight	68 kDa
GenBank accession number	BC056158
Gene Symbol	SREBF2
Gene ID (NCBI)	6721
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q12772
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SREBF2,also named as BHLHD2, is a 1141 amino acid protein, which contains 1 bHLH domain and belongs to the SREBP family. SREBF2 localizes in the endoplasmic reticulum membrane and is ubiquitously expressed in adult and fetal tissues. SREBF2 as a transcriptional activator is required for lipid homeostasis. SREBF2 expression is dynamically regulated in response to nutritional and hormonal cues.

Protocols

Product Specific Protocols
IHC protocol for SREBF2 antibody 14508-1-AP	Download protocol
IP protocol for SREBF2 antibody 14508-1-AP	Download protocol
WB protocol for SREBF2 antibody 14508-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nature The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1. Authors - Jinbo Han View Article
mouse	WB	Nature TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis. Authors - Joanne Hsieh View Article
mouse	WB	Environ Pollut Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet. Authors - Xiaoke Wang View Article
human, mouse	WB	Arterioscler Thromb Vasc Biol RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction. Authors - Yan-Wei Hu View Article
mouse, rat	WB	Arch Toxicol Protective effects of dioscin against alcohol-induced liver injury. Authors - Xu Tingting T View Article
mouse	IHC	J Lipid Res Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein. Authors - Ya-Qi Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 14508-1-AP

WB analysis of HeLa using 14508-1-AP

WB analysis of HEK-293 using 14508-1-AP

WB analysis of HepG2 using 14508-1-AP

WB analysis of mouse kidney using 14508-1-AP

WB analysis of rat kidney using 14508-1-AP

WB analysis of rat liver using 14508-1-AP

WB analysis of human kidney using 14508-1-AP

IP experiment of HeLa using 14508-1-AP

IHC staining of mouse brain using 14508-1-AP

IHC staining of human kidney using 14508-1-AP

IHC staining of human kidney using 14508-1-AP

IHC staining of human liver cancer using 14508-1-AP

IHC staining of human liver cancer using 14508-1-AP

IHC staining of mouse brain using 14508-1-AP

SREBF2 Polyclonal antibody

SREBF2 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The CREB coactivator CRTC2 controls hepatic lipid metabolism by regulating SREBP1.

TTC39B deficiency stabilizes LXR reducing both atherosclerosis and steatohepatitis.

Prenatal exposure to diesel exhaust PM2.5 programmed non-alcoholic fatty liver disease differently in adult male offspring of mice fed normal chow and a high-fat diet.

RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.

Protective effects of dioscin against alcohol-induced liver injury.

Intracellular hepatitis B virus increases hepatic cholesterol deposition in alcoholic fatty liver via hepatitis B core protein.