Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis of A2780 using 24655-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 24655-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A2780 using 24655-1-AP
A2780 cells were subjected to SDS PAGE followed by western blot with 24655-1-AP (SRA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 24655-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 24655-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24655-1-AP (SRA1 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of mouse testis using 24655-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 24655-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24655-1-AP (SRA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 24655-1-AP
Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRA1 antibody (24655-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
FC experiment of MCF-7 using 24655-1-AP
1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug SRA1 Polyclonal antibody (24655-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 24655-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PP7684, SRAP, Steroid receptor RNA activator 1, Steroid receptor RNA activator protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A2780 cells, HEK-293 cells
Positive IHC detected in	human breast cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 1 publications below

Product Information

24655-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20082 Product name: Recombinant human SRA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-209 aa of BC067895 Sequence: GNKERGWNDPPQFSYGLQTQAGGPRRSLLTKRVAAPQDGSPRVPASETSPGPPPMGPPPPSSKAPRSPPVGSGPASGVEPTSFPVESEAVMEDVLRPLEQALEDCRGHTRKQVCDDISRRLALLQEQWAGGKLSIPVKKRMALLVQELSSHRWDAADDIHRSLMVDHVTEVSQWMVGVKRLIAEKRSLF 相同性解析による交差性が予測される生物種
Full Name	steroid receptor RNA activator 1
Calculated molecular weight	26 kDa
Observed molecular weight	30 kDa, 33 kDa
GenBank accession number	BC067895
Gene Symbol	SRA1
Gene ID (NCBI)	10011
RRID	AB_2879657
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9HD15
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

Protocols

Product Specific Protocols
IF protocol for SRA1 antibody 24655-1-AP	Download protocol
IHC protocol for SRA1 antibody 24655-1-AP	Download protocol
WB protocol for SRA1 antibody 24655-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Adv Sci (Weinh) Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway Authors - Ling-Na Zhao View Article
mouse	WB	Free Radic Biol Med Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages Authors - Wenchao Li, View Article
mouse,human	WB	Phytomedicine Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux Authors - Jianfei Mu View Article
mouse,human	WB	Arterioscler Thromb Vasc Biol RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction. Authors - Yan-Wei Hu View Article
human	WB	J Lipid Res A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions. Authors - Yan-Wei Hu View Article
human	WB	J Lipid Res VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet. Authors - Yan-Wei Hu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A2780 using 24655-1-AP

WB analysis of HEK-293 using 24655-1-AP

WB analysis of A2780 using 24655-1-AP

IHC staining of human breast cancer using 24655-1-AP

IHC staining of human breast cancer using 24655-1-AP

IHC staining of mouse testis using 24655-1-AP

IHC staining of mouse testis using 24655-1-AP

IF Staining of MCF-7 using 24655-1-AP

FC experiment of MCF-7 using 24655-1-AP

SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pyruvate Carboxylase in Macrophages Aggravates Atherosclerosis by Regulating Metabolism Reprogramming to Promote Inflammatory Responses Through the Hypoxia-Inducible Factor-1 Signaling Pathway

Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages

Rice bran active peptide (RBAP) inhibited macrophage differentiation to foam cell and atherosclerosis in mice via regulating cholesterol efflux

RP5-833A20.1/miR-382-5p/NFIA-Dependent Signal Transduction Pathway Contributes to the Regulation of Cholesterol Homeostasis and Inflammatory Reaction.

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent Signal Transduction Pathway Is Essential for the Regulation of Cholesterol Homeostasis and Inflammatory Reactions.

VNN1 Promotes Atherosclerosis Progression in apoE Deficiency Mice Fed a High Fat High Cholesterol Diet.