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  • KD/KO Validated

SPRR3 Polyclonal antibody

SPRR3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11742-1-AP

Synonyms

22 kDa pancornulin, Cornifin beta, Esophagin, small proline rich protein 3, SPRC, SPRR3



Tested Applications

Positive WB detected inHeLa cells, human liver tissue
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11742-1-AP targets SPRR3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPRR3 fusion protein Ag2337 相同性解析による交差性が予測される生物種
Full Name small proline-rich protein 3
Calculated molecular weight 19 kDa
Observed molecular weight 22-25 kDa
GenBank accession numberBC017802
Gene symbol SPRR3
Gene ID (NCBI) 6707
RRIDAB_2195272
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPRR3, also named as Esophagin, Cornifin beta and SPRC, belongs to the cornifin (SPRR) family. It is cross-linked envelope protein of keratinocytes. Increased expression of SPRR3 appears to be involved in colorectal tumorigenesis. SPRR3 is a marker for terminal squamous cell differentiation.a potential novel therapeutic target for less advanced stages of Breast Cancer.

Protocols

Product Specific Protocols
WB protocol for SPRR3 antibody 11742-1-APDownload protocol
IHC protocol for SPRR3 antibody 11742-1-APDownload protocol
IF protocol for SPRR3 antibody 11742-1-APDownload protocol
IP protocol for SPRR3 antibody 11742-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouse,ratWB,IP

FASEB J

Small proline-rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts.

Authors - Sarika Saraswati
  • KO Validated
humanWB,IHC

Mol Med

Upregulation of SPRR3 promotes colorectal tumorigenesis.

Authors - Cho Dong-Hyung DH
  • KD Validated
humanWB,IHC

Breast Cancer Res Treat

Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.

Authors - Kim Jin Cheon JC
humanWB

Arch Biochem Biophys

Investigation of mouse amniotic fluid for stimulating ability of keratinocyte differentiation depending on the fetal stage.

Authors - Miki Kuribayashi
humanWB

Onco Targets Ther

Dysregulation of SPRR3/miR-876-3p Axis Contributes to Tumorigenesis in Non-Small-Cell Lung Cancer.

Authors - Qin Li
humanWB

bioRxiv

Discovery of novel microRNA regulators of ribosome biogenesis

Authors - Carson J Bryant
  • KD Validated