• Featured Product
  • KD/KO Validated

SPI1 Polyclonal antibody

SPI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55100-1-AP

Synonyms

31 kDa transforming protein, OF, PU.1, SFPI1, SPI 1, SPI A, SPI1, Transcription factor PU.1



Tested Applications

Positive WB detected inHepG2 cells, RAW264.7, mouse liver tissue, rat liver tissue
Planning an IHC experiment? We recommend our IHCeasy SPI1 Ready-To-Use IHC Kit. SPI1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

55100-1-AP targets SPI1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name spleen focus forming virus (SFFV) proviral integration oncogene spi1
Calculated molecular weight 31 kDa
Observed molecular weight 31-42 kDa
GenBank accession numberNM_003120
Gene symbol SPI1
Gene ID (NCBI) 6688
RRIDAB_10858486
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SPI1 binds to the PU-box which is a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. SPI1 is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B-cells. It also binds RNA and may modulate pre-mRNA splicing. The antibody is specific to SPI1. SPI1 can be detected 37-42 kDa (PMID: 28362429, 31586032, 28586009).

Protocols

Product Specific Protocols
WB protocol for SPI1 antibody 55100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IF

Mucosal Immunol

IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.

Authors - Jiaoyue Hu
mouseWB

Sci Rep

Specific immunotherapy in combination with Clostridium butyricum inhibits allergic inflammation in the mouse intestine.

Authors - Yanhong Shi
humanWB

J Leukoc Biol

miR-199a-5p inhibits monocyte/macrophage differentiation by targeting the activin A type 1B receptor gene and finally reducing C/EBPα expression.

Authors - Hai-Shuang Lin
  • KD Validated
humanWB

Med Oncol

PU.1 negatively regulates tumorigenesis in non-small-cell lung cancer

Authors - Xiaoxiao Liu
humanWB

Exp Cell Res

An innovative immunotherapeutic strategy for rheumatoid arthritis: Selectively suppressing angiogenesis and osteoclast differentiation by fully human antibody targeting thymocyte antigen-1

Authors - Xuanxuan Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Andrew (Verified Customer) (08-09-2021)

When used in our capillary electrophoresis equipment (JESS), the proteintech antibody for SPI1 seemed to show specific binding to a protein at around 60KD. We believe this may be recognising a dimerized form of the protein. This detection was consistent between lanes and responded as expected to varying amounts of sample protein and antibody concentration.

  • Applications: Other
  • Primary Antibody Dilution: Various
  • Cell Tissue Type: Human brain tissue
SPI1 Antibody Other validation (Various dilution) in Human brain tissue (Cat no:55100-1-AP)