SPDEF Polyclonal antibody

Name: SPDEF Polyclonal antibody
Brand: Proteintech
SKU: 11467-1-AP

SPDEF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11467-1-AP

Synonyms

PDEF, Prostate derived Ets factor, Prostate specific Ets, PSE, RP11 375E1__A.3, SPDEF

Validation Data Gallery

Applications:

AllWB

WB analysis of MCF-7 using 11467-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11467-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11467-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11467-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 11467-1-AP (SPDEF Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Cat No. 11467-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in

MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Published Applications

WB	See 8 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11467-1-AP targets SPDEF in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SPDEF fusion protein Ag2020 相同性解析による交差性が予測される生物種
Full Name	SAM pointed domain containing ets transcription factor
Calculated molecular weight	335 aa, 38 kDa
Observed molecular weight	38-44 kDa
GenBank accession number	BC021299
Gene symbol	SPDEF
Gene ID (NCBI)	25803
RRID	AB_2877765
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SPDEF antibody 11467-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell A modular master regulator landscape controls cancer transcriptional identity. Authors - Evan O Paull View Article
human	WB,IHC	Clin Sci (Lond) Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway. Authors - Liping Liang View Article
human	WB,IF	Phytomedicine Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells. Authors - Yu-Hua Lin View Article
mouse	WB	Food Funct EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier. Authors - Jian Fang View Article
mouse	WB	Immunobiology Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway Authors - Yangfan Hou View Article
rat	WB	Dig Dis Sci Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats. Authors - Linhao Zhang View Article

製品

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研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SPDEF Polyclonal antibody

SPDEF Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 11467-1-AP

WB analysis of MCF-7 using 11467-1-AP

WB analysis of MCF-7 using 11467-1-AP

WB analysis of MCF-7 using 11467-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

A modular master regulator landscape controls cancer transcriptional identity.

Gut microbiota-derived butyrate regulates gut mucus barrier repair by activating the macrophage/WNT/ERK signaling pathway.

Resveratrol inhibits MUC5AC expression by regulating SPDEF in lung cancer cells.

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Lactobacillus rhamnosus 76 alleviates airway inflammation in ovalbumin-allergic mice and improves mucus secretion by down-regulating STAT6/SPDEF pathway

Compromised Ileal Mucus Barrier Due to Impaired Epithelial Homeostasis Caused by Notch1 Signaling in Cirrhotic Rats.