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  • Featured Product
  • KD/KO Validated

SPC25 Polyclonal antibody

SPC25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26474-1-AP

Synonyms

AD024, hSpc25, Kinetochore protein Spc25, SPBC25, SPC25

Validation Data Gallery

Applications:
  • WB analysis of THP-1 using 26474-1-AP

    WB analysis of THP-1 using 26474-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 26474-1-AP (SPC25 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 26474-1-AP

    WB analysis of HL-60 using 26474-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 26474-1-AP (SPC25 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 26474-1-AP

    IHC staining of mouse spleen using 26474-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 26474-1-AP

    IHC staining of mouse spleen using 26474-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26474-1-AP (SPC25 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26474-1-AP is SPC25 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SPC25 fusion protein Ag23985 相同性解析による交差性が予測される生物種
Full Name SPC25, NDC80 kinetochore complex component, homolog (S. cerevisiae)
Observed molecular weight 26 kDa
GenBank accession numberBC022255
Gene symbol SPC25
Gene ID (NCBI) 57405
RRIDAB_2880530
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SPC25 antibody 26474-1-APDownload protocol
IHC protocol for SPC25 antibody 26474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Aging (Albany NY)

SPC25 overexpression promotes tumor proliferation and is prognostic of poor survival in hepatocellular carcinoma.

Authors - Baozhu Zhang
View Article
humanWB,IHC

JTO Clin Res Rep

CADM1 and SPC25 Gene Mutations in Lung Cancer Patients With Idiopathic Pulmonary Fibrosis.

Authors - Aya Fukuizumi
  • KD Validated
View Article