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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX18 Monoclonal antibody

SNX18 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E7D6

Cat no : 68305-1-Ig

Synonyms

FLJ11997, FLJ32560, FLJ61062, SH3PX2, SH3PXD3B, SNAG1, SNX18, sorting nexin 18

Validation Data Gallery

Applications:
  • WB analysis using 68305-1-Ig

    WB analysis using 68305-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68305-1-Ig

    WB analysis of NIH/3T3 using 68305-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68305-1-Ig (SNX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68305-1-Ig

    IF Staining of A431 using 68305-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using SNX18 antibody (68305-1-Ig, Clone: 2E7D6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, NIH/3T3 cells, A549 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IF detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68305-1-Ig targets SNX18 in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX18 fusion protein Ag17388 相同性解析による交差性が予測される生物種
Full Name sorting nexin 18
Calculated molecular weight 628 aa, 69 kDa
Observed molecular weight 67-70 kDa
GenBank accession numberBC060791
Gene symbol SNX18
Gene ID (NCBI) 112574
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy, and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
WB protocol for SNX18 antibody 68305-1-IgDownload protocol
IF protocol for SNX18 antibody 68305-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols