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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SNX17 Monoclonal antibody

SNX17 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B8F6

Cat no : 68256-1-Ig

Synonyms

KIAA0064, SNX17, sorting nexin 17

Validation Data Gallery

Applications:
  • WB analysis using 68256-1-Ig

    WB analysis using 68256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68256-1-Ig

    WB analysis using 68256-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 68256-1-Ig

    WB analysis of Jurkat using 68256-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 68256-1-Ig (SNX17 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68256-1-Ig

    IF Staining of HepG2 using 68256-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 68256-1-Ig

    IF Staining of HeLa using 68256-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using SNX17 antibody (68256-1-Ig, Clone: 3B8F6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, pig brain tissue, Jurkat cells, rat brain tissue, HeLa cells
Positive IF detected inHepG2 cells, HeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68256-1-Ig targets SNX17 in WB, IF, ELISA applications and shows reactivity with Human, rat, pig samples.

Tested Reactivity Human, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SNX17 fusion protein Ag32624 相同性解析による交差性が予測される生物種
Full Name sorting nexin 17
Calculated molecular weight 53 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC002524
Gene symbol SNX17
Gene ID (NCBI) 9784
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sorting nexin 17 (SNX17, synonym: KIAA0064) is a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. SNX17 does not contain a coiled coil region, like some family members, but contains a B41 domain. This protein interacts with the cytoplasmic domain of P-selectin, and may function in the intracellular trafficking of P-selectin.

Protocols

Product Specific Protocols
WB protocol for SNX17 antibody 68256-1-IgDownload protocol
IF protocol for SNX17 antibody 68256-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols