Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 25926-1-AP
WB result of SNW1 antibody (25926-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNW1 transfected HepG2 cells.
WB analysis of MCF-7 using 25926-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 25926-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25926-1-AP (SNW1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human prostate cancer using 25926-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 25926-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 25926-1-AP (SNW1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 25926-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SNW1 antibody (25926-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of HepG2 using 25926-1-AP
Immunofluorescent analysis of HepG2 cells using 25926-1-AP (SNW1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SNW1 Polyclonal antibody

SNW1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25926-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

NCOA 62, SKIP, SKIIP, Ski-interacting protein, Nuclear receptor coactivator NCoA-62

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	MCF-7 cells, HepG2 cells
Positive IHC detected in	human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:150-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below

Product Information

25926-1-AP targets SNW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23106 Product name: Recombinant human SNW1 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 188-536 aa of BC108903 Sequence: FNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE 相同性解析による交差性が予測される生物種
Full Name	SNW domain containing 1
Calculated molecular weight	536 aa, 61 kDa
Observed molecular weight	62-69 kDa
GenBank accession number	BC108903
Gene Symbol	SNW1
Gene ID (NCBI)	22938
RRID	AB_2880298
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13573
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for SNW1 antibody 25926-1-AP	Download protocol
IHC protocol for SNW1 antibody 25926-1-AP	Download protocol
WB protocol for SNW1 antibody 25926-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. Authors - Shuai Hou View Article
human	WB	FEBS Lett Split-BioID: a proximity biotinylation assay for dimerization-dependent protein interactions. Authors - Sofie De Munter View Article
human	WB	Microbes Infect SNW1 interacts with IKKγ to positively regulate antiviral innate immune responses against influenza A virus infection. Authors - Qiao Zhang KD Validated View Article
mouse	WB	J Ovarian Res Alteration of the N6-methyladenosine methylation landscape in a mouse model of polycystic ovary syndrome Authors - Lingxiao Zou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 25926-1-AP

WB analysis of MCF-7 using 25926-1-AP

WB analysis of HepG2 using 25926-1-AP

IHC staining of human prostate cancer using 25926-1-AP

IHC staining of human prostate cancer using 25926-1-AP

IF Staining of HepG2 using 25926-1-AP