Validation Data Gallery
Tested Applications
| Positive WB detected in | A375 cells, HEK-293 cells, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human testis tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 12 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
10055-1-AP targets SNAPIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0101 Product name: Recombinant human SNAPIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC000761 Sequence: MAGAGSAAVSGAGTPVAGPTGRDLFAEGLLEFLRPAVQQLDSHVHAVRESQVELREQIDNLATELCRINEDQKVALDLDPYVKKLLNARRRVVLVNNILQNAQERLRRLNHSVAKETARRRAMLDSGIYPPGSPGK 相同性解析による交差性が予測される生物種 |
| Full Name | SNAP-associated protein |
| Calculated molecular weight | 15 kDa |
| Observed molecular weight | 15-18 kDa |
| GenBank accession number | BC000761 |
| Gene Symbol | SNAPIN |
| Gene ID (NCBI) | 23557 |
| RRID | AB_2192656 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95295 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Snapin, a protein of relative molecular weight of 15kd, is implicated in neurotransmission by binding to SNAP-25. Snapin was enriched in neurons and exclusively located on synaptic vesicle membrane, which may be a PKA target for modulating transmitter release through the cAMP-dependent signal-transduction pathway. This anitbody can recognize the 15-18kd(Monomer) and 30-36kd(Dimer) forms of SNAPIN.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SNAPIN antibody 10055-1-AP | Download protocol |
| IP protocol for SNAPIN antibody 10055-1-AP | Download protocol |
| WB protocol for SNAPIN antibody 10055-1-AP | Download protocol |
| FC protocol for SNAPIN antibody 10055-1-AP | Download protocol |
| IF protocol for SNAPIN antibody 10055-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Thromb Haemost Dysregulation of PLDN (pallidin) is a mechanism for platelet dense granule deficiency in RUNX1 haplodeficiency. | ||
Am J Hum Genet A BLOC-1 mutation screen reveals that PLDN is mutated in Hermansky-Pudlak Syndrome type 9. | ||
Autophagy Carbon nanotubes, but not spherical nanoparticles, block autophagy by a shape-related targeting of lysosomes in murine macrophages. | ||
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Autophagy SNAPIN is critical for lysosomal acidification and autophagosome maturation in macrophages. | ||
J Cell Biol LAMTOR/Ragulator is a negative regulator of Arl8b- and BORC-dependent late endosomal positioning. |
