Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human kidney using 12704-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12704-1-AP
WB result of SNAP29 antibody (12704-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNAP29 transfected K-562 cells.
WB analysis of human liver using 12704-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12704-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 12704-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12704-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12704-1-AP (SNAP29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 12704-1-AP
IP result of anti-SNAP29 (IP:12704-1-AP, 4ug; Detection:12704-1-AP 1:600) with Jurkat cells lysate 3200ug.
IHC staining of mouse kidney using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12704-1-AP (SNAP29 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human stomach using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach using 12704-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12704-1-AP (SNAP29 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of PC-12 using 12704-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP29 antibody (12704-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HEK-293 using 12704-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 12704-1-AP (SNAP29 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 12704-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SNAP29 antibody (12704-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SNAP29 Polyclonal antibody

SNAP29 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12704-1-AP

Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

SNAP-29, CEDNIK, SNAP 29, Synaptosomal-associated protein 29, Vesicle-membrane fusion protein SNAP-29

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (34)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human kidney tissue, human liver tissue, HEK-293 cells, Jurkat cells, K-562 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse kidney tissue, human kidney tissue, human stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	PC-12 cells, HEK-293 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 30 publications below
IHC	See 1 publications below
IF	See 3 publications below
IP	See 2 publications below
CoIP	See 1 publications below

Product Information

12704-1-AP targets SNAP29 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3382 Product name: Recombinant human SNAP29 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-258 aa of BC009715 Sequence: MSAYPKSYNPFDDDGEDEGARPAPWRDARDLPDGPDAPADRQQYLRQEVLRRAEATAASTSRSLALMYESEKVGVASSEELARQRGVLERTEKMVDKMDQDLKISQKHINSIKSVFGGLVNYFKSKPVETPPEQNGTLTSQPNNRLKEAISTSKEQEAKYQASHPNLRKLDDTDPVPRGAGSAMSTDAYPKNPHLRAYHQKIDSNLDELSMGLGRLKDIALGMQTEIEEQDDILDRLTTKVDKLDVNIKSTERKVRQL 相同性解析による交差性が予測される生物種
Full Name	synaptosomal-associated protein, 29kDa
Calculated molecular weight	258 aa, 29 kDa
Observed molecular weight	29 kDa
GenBank accession number	BC009715
Gene Symbol	SNAP29
Gene ID (NCBI)	9342
RRID	AB_2192340
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95721
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SNAREs, soluble N-ethylmaleimide-sensitive factor-attachment protein receptors, are essential proteins for the fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. SNAP29 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysosome membrane. SNAP29 also plays a role in ciliogenesis by regulating membrane fusions.

Protocols

Product Specific Protocols
IF protocol for SNAP29 antibody 12704-1-AP	Download protocol
IHC protocol for SNAP29 antibody 12704-1-AP	Download protocol
IP protocol for SNAP29 antibody 12704-1-AP	Download protocol
WB protocol for SNAP29 antibody 12704-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
human	WB	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
human	WB	Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes Authors - Wang Zheng View Article
human	WB	Nat Commun Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice. Authors - Ting Li View Article
human	WB,IF	Nat Commun KRAS regulation by small non-coding RNAs and SNARE proteins. Authors - Yonglu Che View Article
human		Autophagy The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication. Authors - Peili Hou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human kidney using 12704-1-AP

WB analysis of K-562 using 12704-1-AP

WB analysis of human liver using 12704-1-AP

WB analysis of Jurkat using 12704-1-AP

WB analysis of K-562 using 12704-1-AP

WB analysis of HEK-293 using 12704-1-AP

IP experiment of Jurkat using 12704-1-AP

IHC staining of mouse kidney using 12704-1-AP

IHC staining of mouse kidney using 12704-1-AP

IHC staining of human stomach using 12704-1-AP

IHC staining of human kidney using 12704-1-AP

IHC staining of human kidney using 12704-1-AP

IHC staining of human stomach using 12704-1-AP

IHC staining of human stomach using 12704-1-AP

IF Staining of PC-12 using 12704-1-AP

IF Staining of HEK-293 using 12704-1-AP

IF Staining of HeLa using 12704-1-AP

SNAP29 Polyclonal antibody

SNAP29 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Kansl1 haploinsufficiency impairs autophagosome-lysosome fusion and links autophagic dysfunction with Koolen-de Vries syndrome in mice.

KRAS regulation by small non-coding RNAs and SNARE proteins.

The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.