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Species Reactivity
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Species Reactivity
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SMURF1 Monoclonal antibody

SMURF1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A7E4

Cat no : 68466-1-Ig

Synonyms

hSMURF1, KIAA1625, SMURF1

Validation Data Gallery

Applications:
  • WB analysis using 68466-1-Ig

    WB analysis using 68466-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68466-1-Ig (SMURF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, A549 cells, U2OS cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68466-1-Ig targets SMURF1 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SMURF1 fusion protein Ag30891 相同性解析による交差性が予測される生物種
Full Name SMAD specific E3 ubiquitin protein ligase 1
Calculated molecular weight 86 kDa
Observed molecular weight86 kDa
GenBank accession numberNM_020429
Gene symbol SMURF1
Gene ID (NCBI) 57154
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMURF1, also named as KIAA1625, is E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. SMURF1 interacts with receptor-regulated SMADs specific for the BMP pathway, SMAD1 and SMAD5, in order to trigger their ubiquitination and degradation and hence their inactivation. SMURF1 can be detected as 90-100 kDa when ubiquitinated (PMID:17576816, PMID:20484049).

Protocols

Product Specific Protocols
WB protocol for SMURF1 antibody 68466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols