Validation Data Gallery
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Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293 cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
16552-1-AP targets SMPDL3B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, monkey, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SMPDL3B fusion protein Ag9535 相同性解析による交差性が予測される生物種 |
| Full Name | sphingomyelin phosphodiesterase, acid-like 3B |
| Calculated molecular weight | 373aa,42 kDa; 455aa,51 kDa |
| Observed molecular weight | 42 kDa, 50-65 kDa |
| GenBank accession number | BC014444 |
| Gene Symbol | SMPDL3B |
| Gene ID (NCBI) | 27293 |
| RRID | AB_2878276 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92485 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SMPDL3B is lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity. SMPDL3B acts as a negative regulator of Toll-like receptor signaling.It lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP in vitro (PMID: 26095358). SMPDL3B can be detected 60-70 kDa by N-glycosylation modification (PMID: 26095358, PMID: 31217420).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
| IHC protocol for SMPDL3B antibody 16552-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Mol Biosci SMPDL3B Predicts Poor Prognosis and Contributes to Development of Acute Myeloid Leukemia
| ||
JHEP Rep Macrophage-derived thrombospondin 1 promotes obesity-associated non-alcoholic fatty liver disease. | ||
iScience Porcine reproductive and respiratory syndrome virus upregulates SMPDL3B to promote viral replication by modulating lipid metabolism
|