Validation Data Gallery

Filter: AllWBIHC

WB analysis of rat brain/liver tissue using 14195-1-AP
WB result of 14195-1-AP from Dilwar in Rat tissue. Rat brain (a), heart (b) liver (c) mitochondria and liver homogenate (d), (40 ug with 10% PAGE) probed with 1:1000 dilution.
WB analysis using 14195-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 14195-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 14195-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14195-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 14195-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human osteosarcoma using 14195-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (CAT-1 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human osteosarcoma using 14195-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14195-1-AP (SLC7A1/CAT-1 antibody) at dilution of 1:100 (under 10x lens).

Featured Product
KD/KO Validated

SLC7A1/CAT-1 Polyclonal antibody

SLC7A1/CAT-1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14195-1-AP

Publications(34)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

CAT-1, SLC7A1, CAT1, ERR

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (34)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, Jurkat cells, HepG2 cells, rat brain/liver tissue, mouse heart tissue, rat liver tissue
Positive IHC detected in	human osteosarcoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 22 publications below
IHC	See 12 publications below
IF	See 4 publications below
CoIP	See 1 publications below

Product Information

14195-1-AP targets SLC7A1/CAT-1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5398 Product name: Recombinant human SLC7A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 312-629 aa of BC063303 Sequence: MPYFCLDNNSPLPDAFKHVGWEGAKYAVAVGSLCALSASLLGSMFPMPRVIYAMAEDGLLFKFLANVNDRTKTPIIATLASGAVAAVMAFLFDLKDLVDLMSIGTLLAYSLVAACVLVLRYQPEQPNLVYQMASTSDELDPADQNELASTNDSQLGFLPEAEMFSLKTILSPKNMEPSKISGLIVNISTSLIAVLIITFCIVTVLGREALTKGALWAVFLLAGSALLCAVVTGVIWRQPESKTKLSFKVPFLPVLPILSIFVNVYLMMQLDQGTWVRFAVWMLIGFIIYFGYGLWHSEEASLDADQARTPDGNLDQCK 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 7 (cationic amino acid transporter, y+ system), member 1
Calculated molecular weight	68 kDa
Observed molecular weight	67-70 kDa
GenBank accession number	BC063303
Gene Symbol	CAT-1
Gene ID (NCBI)	6541
RRID	AB_2190723
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P30825
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for SLC7A1/CAT-1 antibody 14195-1-AP	Download protocol
WB protocol for SLC7A1/CAT-1 antibody 14195-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions. Authors - Hongling Huang KO Validated View Article
mouse	IHC	J Cachexia Sarcopenia Muscle Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction. Authors - Thomas Kunzke View Article
		Free Radic Biol Med Cold plasma-treated medium preferentially eliminates doxorubicin-resistant osteosarcoma cells Authors - Juan Tornín View Article
Rat		Oxid Med Cell Longev l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways. Authors - Li Zeng View Article
mouse,human	WB	J Steroid Biochem Mol Biol High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1. Authors - Rui Wang View Article
human	WB	Mol Cell Proteomics Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression. Authors - James R Byrnes View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat brain/liver tissue using 14195-1-AP

WB analysis using 14195-1-AP

WB analysis of mouse liver using 14195-1-AP

WB analysis of mouse heart using 14195-1-AP

WB analysis of HepG2 using 14195-1-AP

WB analysis of Jurkat using 14195-1-AP

IHC staining of human osteosarcoma using 14195-1-AP

IHC staining of human osteosarcoma using 14195-1-AP

SLC7A1/CAT-1 Polyclonal antibody

SLC7A1/CAT-1 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions.

Derangements of amino acids in cachectic skeletal muscle are caused by mitochondrial dysfunction.

Cold plasma-treated medium preferentially eliminates doxorubicin-resistant osteosarcoma cells

l-Theanine Ameliorates d-Galactose-Induced Brain Damage in Rats via Inhibiting AGE Formation and Regulating Sirtuin1 and BDNF Signaling Pathways.

High glucose impaired estrogen receptor alpha signaling via β-catenin in osteoblastic MC3T3-E1.

Hypoxia is a dominant remodeler of the effector T cell surface proteome relative to activation and regulatory T cell suppression.