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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis using 67700-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis using 67700-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HSC-T6 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    WB analysis of HSC-T6 using 67700-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    IF Staining of H9C2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using SLC6A12 Monoclonal antibody (67700-1-Ig)

    FC experiment of HepG2 using 67700-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.2 μg SLC6A12 Monoclonal antibody (67700-1-Ig, Clone:2C1G4) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005)(N/A), or 0.2 μg KLH (66360-2-Ig, Clone: K11A1B2A2) (N/A). Cells were fixed with 4% PFA .

SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67700-1-Ig
Clone No.2C1G4
Publications(3)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IF/ICC, ELISA

Synonyms

BGT 1, BGT1, FLJ38727, SLC6A12

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, rat liver tissue, HSC-T6 cells, HuH-7 cells, HepG2 cells, mouse hepatocytes
Positive IF/ICC detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

67700-1-Ig targets SLC6A12 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivityhuman, mouse, canine
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag18962

Product name: Recombinant human SLC6A12 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 548-614 aa of BC126215

Sequence: VCVPLFVVITLLKTRGPFRKRLRQLITPDSSLPQPKQHPCLDGSAGRNFGPSPTREGLIAGEKETHL

相同性解析による交差性が予測される生物種
Full Name solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Calculated molecular weight 614 aa, 69 kDa
Observed molecular weight70 kDa
GenBank accession numberBC126215
Gene Symbol SLC6A12
Gene ID (NCBI) 6539
RRIDAB_2882892
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP48065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for SLC6A12 antibody 67700-1-IgDownload protocol
WB protocol for SLC6A12 antibody 67700-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Am J Physiol Renal Physiol

Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress.

Authors - Eva S Schweikhard
View Article
mouseWB

Int J Biol Sci

Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12

Authors - Xianzheng Zhang
View Article
humanWB

Dev Cell

The de novo synthesis of GABA and its gene regulatory function control hepatocellular carcinoma metastasis

Authors - Li Li
View Article
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