Sodium iodide symporter Polyclonal antibody
Sodium iodide symporter Polyclonal Antibody for WB, IP, FC, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, FC, IHC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 24324-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, mouse stomach tissue, SGC-7901 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human thyroid cancer tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 25 publications below |
| IHC | See 5 publications below |
| IF | See 13 publications below |
| IP | See 1 publications below |
| FC | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
The immunogen of 24324-1-AP is Sodium iodide symporter Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Sodium iodide symporter fusion protein Ag19504 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 5 (sodium iodide symporter), member 5 |
| Calculated molecular weight | 643 aa, 69 kDa |
| Observed molecular weight | 50-55 kDa, 75-100 kDa |
| GenBank accession number | BC105047 |
| Gene symbol | SLC5A5 |
| Gene ID (NCBI) | 6528 |
| RRID | AB_2879495 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The sodium iodide symporter (Na+/I - symporter, NIS), encoded by SLC5A5, is an integral plasma membrane glycoprotein that plays an important role in iodide uptake by thyroid cells. Expression of sodium iodide symporter has also been found in extra-thyroidal tissues, including gastric mucosa, lactating mammary gland and salivary glands. Increased expression of sodium iodide symporter has been found in thyroid tissue from patients with Graves' disease as well as papillary thyroid carcinomas. In addition, sodium iodide symporter was found to express in majority of breast cancer tissue but not in normal tissue. Sodium iodide symporter can be a promising diagnostic and therapeutic tool for thyroid cancer and breast cancer. This antibody recognizes the mature approximately 75-100 kDa protein and a partially glycosylated 50-55 kDa protein. (PMID: 12588808, 9525971)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Sodium iodide symporter antibody 24324-1-AP | Download protocol |
| IHC protocol for Sodium iodide symporter antibody 24324-1-AP | Download protocol |
| IP protocol for Sodium iodide symporter antibody 24324-1-AP | Download protocol |
| FC protocol for Sodium iodide symporter antibody 24324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice | ||
Cell Death Differ REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway. | ||
Cell Death Differ CRSP8 promotes thyroid cancer progression by antagonizing IKKα-induced cell differentiation. | ||
Cell Chem Biol Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter. | ||
Cancer Lett IGF2BP2-dependent activation of ERBB2 signaling contributes to acquired resistance to tyrosine kinase inhibitor in differentiation therapy of radioiodine-refractory papillary thyroid cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Katie (Verified Customer) (08-22-2019) | Highly specific antibody for the sodium iodide symporter (NIS) with no background bands in Western blot, used at 1:1000 dilution. Very clear result. Good value for money, and excellent communication prior to delivery receipt.
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