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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Band 3 / AE1 Recombinant antibody

Band 3 / AE1 Recombinant Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3K21

Cat no : 81066-1-RR

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, Band 3 anion transport protein, BND3, CD233, DI, EMPB3, EPB3, FR, RTA1A, SLC4A1, SW, WD, WD1, WR

Validation Data Gallery

Applications:
  • IHC staining of human placenta using 81066-1-RR

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Band 3/AE1 antibody included
  • IHC staining of human placenta using 81066-1-RR

    IHC staining of human placenta using 81066-1-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 81066-1-RR (SLC4A1 antibody) at dilution of 1:1700 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Band 3/AE1 antibody included
  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human placenta using 81066-1-RR

    IF Staining of human placenta using 81066-1-RR

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using Band 3/ AE1 antibody (81066-1-RR, Clone: 3K21 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

81066-1-RR targets Band 3 / AE1 in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Band 3 / AE1 fusion protein Ag27878 相同性解析による交差性が予測される生物種
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
GenBank accession numberBC101570
Gene symbol SLC4A1
Gene ID (NCBI) 6521
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

Protocols

Product Specific Protocols
IHC protocol for Band 3 / AE1 antibody 81066-1-RRDownload protocol
IF protocol for Band 3 / AE1 antibody 81066-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols