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  • KD/KO Validated

ZIP8 Polyclonal antibody

ZIP8 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 20459-1-AP

Synonyms

BIGM103, PP3105, SLC39A8, ZIP 8, ZIP8



Tested Applications

Positive WB detected inmouse lung tissue, mouse heart tissue, U-937 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue, human placenta tissue, mouse lung tissue, mouse small intestine tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20459-1-AP is ZIP8 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZIP8 fusion protein Ag14292 相同性解析による交差性が予測される生物種
Full Name solute carrier family 39 (zinc transporter), member 8
Calculated molecular weight 460 aa, 50 kDa
Observed molecular weight 42-46 kDa, 75-90 kDa
GenBank accession numberBC012125
Gene symbol SLC39A8
Gene ID (NCBI) 64116
RRIDAB_10697830
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SLC39A8, also known as ZIP8, belongs to the ZIP family of metal ion transporters which function to transport zinc and/or other metal ion substrates from the extracellular space or organellar lumen into the cytoplasm. Recently it was found that ZIP8 expression is upregulated in human monocytes in response to LPS, TNF-α, and live bacteria, facilitating cytoprotection during the early inflammation. Besides zinc ZIP8 can also transport cadmium and manganese efficiently. It is predicted that ZIP8 contains 3 potential N-linked glycosylation sites and is subject to glycosylation, which may account for the presences of multiple molecular weights, such as 43 kDa, 49 kDa, 60 kDa, 75-90 kDa, 150 kDa, and 200 kDa.

Protocols

Product Specific Protocols
WB protocol for ZIP8 antibody 20459-1-APDownload protocol
IHC protocol for ZIP8 antibody 20459-1-APDownload protocol
IP protocol for ZIP8 antibody 20459-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Cell

Regulation of the Catabolic Cascade in Osteoarthritis by the Zinc-ZIP8-MTF1 Axis.

Authors - Jin-Hong Kim
mouseWB,IF

J Clin Invest

Hepatic metal ion transporter ZIP8 regulates manganese homeostasis and manganese-dependent enzyme activity.

Authors - Wen Lin
  • KO Validated
mouseWB

Acta Neuropathol Commun

Deregulation of subcellular biometal homeostasis through loss of the metal transporter, Zip7, in a childhood neurodegenerative disorder.

Authors - Alexandra Grubman
ratWB

Int J Mol Sci

Mitochondria-Targeting Antioxidant Provides Cardioprotection through Regulation of Cytosolic and Mitochondrial Zn2+ Levels with Re-Distribution of Zn2+-Transporters in Aged Rat Cardiomyocytes.

Authors - Yusuf Olgar
mouseWB,IF

Int Immunopharmacol

ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx.

Authors - Gong Cheng
  • KD Validated
humanIHC

Front Cell Dev Biol

An Iron Metabolism-Related Gene Signature for the Prognosis of Colon Cancer.

Authors - Jing Yuan
  • KD Validated