Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 12943-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human testis using 12943-1-AP
human testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12943-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12943-1-AP (SLC27A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 12943-1-AP
IP result of anti-SLC27A3 (IP:12943-1-AP, 4ug; Detection:12943-1-AP 1:500) with mouse testis tissue lysate 4000ug.
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skin using 12943-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 12943-1-AP (SLC27A3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SLC27A3 Polyclonal antibody

SLC27A3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 12943-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Synonyms

ACSVL3, FATP 3, FATP3, Fatty acid transport protein 3, SLC27A3, UNQ367/PRO703, VLCS 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human testis tissue, mouse testis tissue, MCF-7 cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human skin cancer tissue, human breast cancer tissue, mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 6 publications below
IHC	See 1 publications below

Product Information

12943-1-AP targets SLC27A3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4015 Product name: Recombinant human SLC27A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 500-700 aa of BC029792 Sequence: GEPGLLVAPVSQQSPFLGYAGGPELAQGKLLKDVFRPGDVFFNTGDLLVCDDQGFLRFHDRTGDTFRWKGENVATTEVAEVFEALDFLQEVNVYGVTVPGHEGRAGMAALVLRPPHALDLMQLYTHVSENLPPYARPRFLRLQESLATTETFKQQKVRMANEGFDPSTLSDPLYVLDQAVGAYLPLTTARYSALLAGNLRI 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 27 (fatty acid transporter), member 3
Calculated molecular weight	75 kDa
Observed molecular weight	70-79 kDa
GenBank accession number	BC029792
Gene Symbol	SLC27A3
Gene ID (NCBI)	11000
RRID	AB_2286200
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5K4L6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SLC27A3, also named as FATP3, is a member of the solute carrier 27A (SLC27A) gene family which encodes fatty acid transport proteins. SLC27A3 is expressed in human neural stem cells and plays a key role in the developmental stage of the central nervous system. It is associated with autism spectrum disorders (ASD) that caused by impaired early brain development.(PMID: 26548558).

Protocols

Product Specific Protocols
IHC protocol for SLC27A3 antibody 12943-1-AP	Download protocol
IP protocol for SLC27A3 antibody 12943-1-AP	Download protocol
WB protocol for SLC27A3 antibody 12943-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Med PMID: 26950361 Authors - Cholsoon Jang KD Validated View Article
mouse	WB	Cancers (Basel) In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer. Authors - Megan C Madonna View Article
mouse	WB	Eur J Pharmacol A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate Authors - Yuanli Chen View Article
human,mouse	WB,IHC	Front Immunol Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Authors - Yuepeng Zhang View Article
pig	WB	Mol Reprod Dev Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport. Authors - Zheng Ao View Article
mouse	WB	Int J Mol Sci Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development Authors - Mingfa Ling View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 12943-1-AP

WB analysis of human testis using 12943-1-AP

WB analysis of mouse testis using 12943-1-AP

IP experiment of mouse testis using 12943-1-AP

IHC staining of human skin cancer using 12943-1-AP

IHC staining of human skin cancer using 12943-1-AP

IHC staining of human skin cancer using 12943-1-AP

IHC staining of human skin cancer using 12943-1-AP

IHC staining of human breast cancer using 12943-1-AP

IHC staining of human breast cancer using 12943-1-AP

IHC staining of human breast cancer using 12943-1-AP

IHC staining of human breast cancer using 12943-1-AP

IHC staining of mouse skin using 12943-1-AP

IHC staining of mouse skin using 12943-1-AP

SLC27A3 Polyclonal antibody

SLC27A3 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PMID: 26950361

In Vivo Optical Metabolic Imaging of Long-Chain Fatty Acid Uptake in Orthotopic Models of Triple-Negative Breast Cancer.

A novel therapy for hepatic cholestasis treatment-the combination of rosiglitazone and fenofibrate

Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease.

Cloned pig fetuses exhibit fatty acid deficiency from impaired placental transport.

Knockdown of VEGFB/VEGFR1 Signaling Promotes White Adipose Tissue Browning and Skeletal Muscle Development