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NKCC1,SLC12A2 Polyclonal antibody
NKCC1,SLC12A2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 13884-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, 37°C incubated mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 9 publications below |
| IHC | See 9 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
13884-1-AP targets NKCC1,SLC12A2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | NKCC1,SLC12A2 fusion protein Ag4991 相同性解析による交差性が予測される生物種 |
| Full Name | solute carrier family 12 (sodium/potassium/chloride transporters), member 2 |
| Calculated molecular weight | 131 kDa |
| Observed molecular weight | 150-160 kDa |
| GenBank accession number | BC033003 |
| Gene symbol | SLC12A2 |
| Gene ID (NCBI) | 6558 |
| RRID | AB_2188522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for NKCC1,SLC12A2 antibody 13884-1-AP | Download protocol |
| IHC protocol for NKCC1,SLC12A2 antibody 13884-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
PLoS Biol Early uneven ear input induces long-lasting differences in left-right motor function. | ||
PLoS Biol The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner. | ||
J Neurosci The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety. | ||
Front Mol Neurosci Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampus | ||
Acta Physiol (Oxf) Bile deficiency induces changes of intestinal Cl(-) and HCO3 (-) secretions in mice. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Valentina (Verified Customer) (02-01-2023) | Multiple bands were detectable around 100 - 150 kDa, with different protocols and gels. The antibody is definitely working even if not optimally, probably necessary to try with a higher dilution and a lower amount of protein.
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