Validation Data Gallery

Filter: AllWBIHCIF

WB analysis of HEK-293 using 18970-1-AP
WB result of NKCC2 antibody (18970-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NKCC2 transfected HEK-293 cells.
WB analysis using 18970-1-AP
Boiled or non-boiled lysates of mouse kidney were subjected to SDS PAGE followed by western blot with 18970-1-AP (NKCC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 18970-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18970-1-AP (NKCC2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse Embryonic kidney tissue using 18970-1-AP
IF rsult of SLC12A1 antibody (18970-1-AP, 1:900) with mouse Embryonic kidneys from E16.5 (fixed with 4% PFA at 4C for 30 min, cryosections 5% goat serum/PBST was used for blocking and antibody incubation). Red: anti-rabbit Alexa555, Blue: Hoechst.

Featured Product
KD/KO Validated

SLC12A1/NKCC2 Polyclonal antibody

SLC12A1/NKCC2 Polyclonal Antibody for WB, IHC, IF, ELISA

Cat No. 18970-1-AP

Publications(32)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

NKCC2, SLC12A1, BSC1, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Kidney-specific Na-K-Cl symporter

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (32)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, 37°C incubated mouse kidney tissue
Positive IHC detected in	human kidney tissue, mouse kidney tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	mouse Embryonic kidney tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:2000-1:8000
Immunofluorescence (IF)	IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below
IHC	See 7 publications below
IF	See 22 publications below

Product Information

18970-1-AP targets SLC12A1/NKCC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5325 Product name: Recombinant human SLC12A1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-176 aa of BC040138 Sequence: MSLNNSSNVFLDSVPSNTNRFQVSVINENHESSAAADDNTDPPHYEETSFGDEAQKRLRISFRPGNQECYDNFLQSGETAKTDASFHAYDSHTNTYYLQTFGHNTMDAVPKIEYYRNTGSISGPKVNRPSLLEIHEQLAKNVAVTPSSADRVANGDGIPGDEQAENKEDDQAGVVK 相同性解析による交差性が予測される生物種
Full Name	solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Calculated molecular weight	121 kDa
Observed molecular weight	135 kDa
GenBank accession number	BC040138
Gene Symbol	SLC12A1/NKCC2
Gene ID (NCBI)	6557
RRID	AB_2877130
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13621
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for SLC12A1/NKCC2 antibody 18970-1-AP	Download protocol
WB protocol for SLC12A1/NKCC2 antibody 18970-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human		Cell Stem Cell Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk. Authors - Sara E Howden View Article
	IF	Nat Protoc Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells Authors - Jessica M Vanslambrouck View Article
human	IF	Nat Commun Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids Authors - Jessica M. Vanslambrouck View Article
human	IF	J Am Soc Nephrol A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids. Authors - Jessica M Vanslambrouck View Article
mouse	IF	J Am Soc Nephrol Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney. Authors - Sierra S Marable View Article
mouse,human	WB,IHC	Mol Ther Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene. Authors - Bin Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 18970-1-AP

WB analysis using 18970-1-AP

IHC staining of human kidney using 18970-1-AP

IHC staining of mouse kidney using 18970-1-AP

IHC staining of mouse kidney using 18970-1-AP

IHC staining of human kidney using 18970-1-AP

IHC staining of rat kidney using 18970-1-AP

IHC staining of rat kidney using 18970-1-AP

IF Staining of mouse Embryonic kidney tissue using 18970-1-AP

SLC12A1/NKCC2 Polyclonal antibody

SLC12A1/NKCC2 Polyclonal Antibody for WB, IHC, IF, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Plasticity of distal nephron epithelia from human kidney organoids enables the induction of ureteric tip and stalk.

Generation of proximal tubule-enhanced kidney organoids from human pluripotent stem cells

Enhanced metanephric specification to functional proximal tubule enables toxicity screening and infectious disease modelling in kidney organoids

A Toolbox to Characterize Human Induced Pluripotent Stem Cell-Derived Kidney Cell Types and Organoids.

Hnf4a Is Required for the Development of Cdh6-Expressing Progenitors into Proximal Tubules in the Mouse Kidney.

Tubular-specific CDK12 knockout causes a defect in urine concentration due to premature cleavage of the slc12a1 gene.