Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, mouse kidney tissue, mouse colon tissue, rat colon tissue |
| Positive IHC detected in | human liver cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 45 publications below |
| IHC | See 9 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
| CoIP | See 2 publications below |
Product Information
15168-1-AP targets SIGMAR1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.
| Tested Reactivity | human, mouse, rat, zebrafish |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7351 Product name: Recombinant human SIGMAR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-223 aa of BC004899 Sequence: SEYVLLFGTALGSRGHSGRYWAEISDTIISGTFHQWREGTTKSEVFYPGETVVHGPGEATAVEWGPNTWMVEYGRGVIPSTLAFALADTVFSTQDFLTLFYTLRSYARGLRLELTTYLFGQDP 相同性解析による交差性が予測される生物種 |
| Full Name | sigma non-opioid intracellular receptor 1 |
| Calculated molecular weight | 25 kDa |
| Observed molecular weight | 27 kDa |
| GenBank accession number | BC004899 |
| Gene Symbol | SIGMAR1 |
| Gene ID (NCBI) | 10280 |
| RRID | AB_2301712 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99720 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 28 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIGMAR1 antibody 15168-1-AP | Download protocol |
| WB protocol for SIGMAR1 antibody 15168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. | ||
Ann Neurol Hereditary Spastic Paraplegia-Linked REEP1 Modulates ER-Mitochondria Contacts. | ||
Proc Natl Acad Sci U S A DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1. | ||
Schizophr Bull Allosteric Modulation of the Sigma-1 Receptor Elicits Antipsychotic-like Effects. | ||
Cell Mol Life Sci PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis. | ||
Int J Mol Sci Sigma-1 Receptor Is Critical for Mitochondrial Activity and Unfolded Protein Response in Larval Zebrafish.
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