Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, HEK-293T cells, Jurkat cells, mouse colon tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
55352-1-AP targets SIDT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SID1 transmembrane family, member 1 |
| Calculated molecular weight | 94 kDa |
| Observed molecular weight | 95-110 kDa |
| GenBank accession number | NM_017699 |
| Gene Symbol | SIDT1 |
| Gene ID (NCBI) | 54847 |
| RRID | AB_11182721 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NXL6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SIDT1, also known as a human orthologue of SID-1, belongs to the SID family which is a highly conserved group of transmembrane channel-like proteins. As a transmembrane channel for intercellular communication, SIDT1 was found to facilitate small interfering RNA (siRNA) or miRNA uptake and also involved in cholesterol transport in human systems. SIDT1 mRNA and protein were mainly detected in the stomach and large intestine (including the cecum, colon and rectum) but not in the small intestine (especially the duodenum). Notably, SIDT1 was mainly located in gastric surface mucous cells (pit cells) but barely expressed in the parietal or chief cells. The SIDT1 gene encodes an 827 amino acid protein with a calculated molecular mass of 94 kDa. (PMID: 31632087, PMID: 32801357, PMID: 22174421)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIDT1 antibody 55352-1-AP | Download protocol |
| IHC protocol for SIDT1 antibody 55352-1-AP | Download protocol |
| IF protocol for SIDT1 antibody 55352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Onco Targets Ther Integrated Bioinformatics Data Analysis Reveals Prognostic Significance Of SIDT1 In Triple-Negative Breast Cancer. |