Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, DU 145 cells, HEK-293 cells, human brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 4 publications below |
| IP | See 2 publications below |
Product Information
14649-1-AP targets POSH in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6243 Product name: Recombinant human SH3RF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 696-1056 aa of BC053671 Sequence: VTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI 相同性解析による交差性が予測される生物種 |
| Full Name | SH3 domain containing ring finger 1 |
| Calculated molecular weight | 93 kDa |
| Observed molecular weight | 93-100 kDa |
| GenBank accession number | BC053671 |
| Gene Symbol | SH3RF1/POSH |
| Gene ID (NCBI) | 57630 |
| RRID | AB_2187290 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z6J0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for POSH antibody 14649-1-AP | Download protocol |
| IP protocol for POSH antibody 14649-1-AP | Download protocol |
| WB protocol for POSH antibody 14649-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Biol Rab8, POSH, and TAK1 regulate synaptic growth in a Drosophila model of frontotemporal dementia. | ||
Int J Mol Sci Dual Leucine Zipper Kinase Is Constitutively Active in the Adult Mouse Brain and Has Both Stress-Induced and Homeostatic Functions. | ||
Hum Mol Genet The Pro-Apoptotic JNK Scaffold POSH/SH3RF1 Mediates CHMP2BIntron5-Associated Toxicity in Animal Models of Frontotemporal Dementia. | ||
Expert Opin Ther Targets POSH participates in epileptogenesis by increasing the surface expression of the NMDA receptor: a promising therapeutic target for epilepsy.
| ||
Cell Rep POSH regulates assembly of the NMDAR/PSD-95/Shank complex and synaptic function. | ||
Int J Biol Macromol Snakehead vesiculovirus hijacks SH3RF1 for replication via mediating K63-linked ubiquitination at K264 of the phosphoprotein |
