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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using SGCG Polyclonal antibody (18102-1-AP)

    WB analysis of human skeletal muscle using 18102-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SGCG Polyclonal antibody (18102-1-AP)

    WB analysis using 18102-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using SGCG Polyclonal antibody (18102-1-AP)

    WB analysis of mouse heart using 18102-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using SGCG Polyclonal antibody (18102-1-AP)

    WB analysis of human heart using 18102-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18102-1-AP (SGCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using SGCG Polyclonal antibody (18102-1-AP)

    IP experiment of mouse heart using 18102-1-AP

    IP result of anti-SGCG (IP:18102-1-AP, 4ug; Detection:18102-1-AP 1:1000) with mouse heart tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human heart tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human heart using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human heart using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human kidney using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human kidney using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human placenta using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human placenta using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human skin using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human skin using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human lung using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human lung using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human ovary using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using SGCG Polyclonal antibody (18102-1-AP)

    IHC staining of human ovary using 18102-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18102-1-AP (SGCG antibody) at dilution of 1:50 (under 40x lens).

SGCG Polyclonal antibody

SGCG Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18102-1-AP
Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

35 kDa dystrophin-associated glycoprotein, Gamma sarcoglycan, Gamma-sarcoglycan, Gamma-SG

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, human skeletal muscle tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18102-1-AP targets SGCG in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12402

Product name: Recombinant human SGCG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-291 aa of BC074777

Sequence: MVREQYTTATEGICIERPENQYVYKIGIYGWRKRCLYLFVLLLLIILVVNLALTIWILKVMWFSPAGMGHLCVTKDGLRLEGESEFLFPLYAKEIHSRVDSSLLLQSTQNVTVNARNSEGEVTGRLKVGPKMVEVQNQQFQINSNDGKPLFTVDEKEVVVGTDKLRVTGPEGALFEHSVETPLVRADPFQDLRLESPTRSLSMDAPRGVHIQAHAGKIEALSQMDILFHSSDGMLVLDAETVCLPKLVQGTWGPSGSSQSLYEICVCPDGKLYLSVAGVSTTCQEHSHICL

相同性解析による交差性が予測される生物種
Full Name sarcoglycan, gamma (35kDa dystrophin-associated glycoprotein)
Calculated molecular weight 291 aa, 32 kDa
Observed molecular weight 35-38 kDa
GenBank accession numberBC074777
Gene Symbol SGCG
Gene ID (NCBI) 6445
RRIDAB_2286186
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13326
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for SGCG antibody 18102-1-APDownload protocol
IP protocol for SGCG antibody 18102-1-APDownload protocol
WB protocol for SGCG antibody 18102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP,IF

Skelet Muscle

Novel γ-sarcoglycan interactors in murine muscle membranes.

Authors - Tara C Smith
View Article
mouseIHC

PLoS One

Peptide-conjugated phosphodiamidate oligomer-mediated exon skipping has benefits for cardiac function in mdx and Cmah-/-mdx mouse models of Duchenne muscular dystrophy.

Authors - Alison M Blain
View Article
humanIF

PLoS One

Correlation of Utrophin Levels with the Dystrophin Protein Complex and Muscle Fibre Regeneration in Duchenne and Becker Muscular Dystrophy Muscle Biopsies.

Authors - Narinder Janghra
View Article
humanIF

Hum Gene Ther Methods

Optimisation of internally deleted dystrophin constructs.

Authors - Mojgan Reza
View Article
mouseIHC

Mol Vis

Identification of HMX1 target genes: a predictive promoter model approach.

Authors - Boulling Arnaud A
View Article
mouseWB

Adv Sci (Weinh)

Sirt6 Mono-ADP-Ribosylates YY1 to Promote Dystrophin Expression for Neuromuscular Transmission

Authors - Wei Zhang
View Article
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