Validation Data Gallery
Tested Applications
| Positive WB detected in | pig brain tissue, Y79 cells, COLO 320 cells, rat brain tissue, rabbit brain tissue, mouse brain tissue |
| Positive IHC detected in | human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
Product Information
66328-1-Ig targets SFRP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18840 Product name: Recombinant human SFRP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-295 aa of BC008666 Sequence: MLQGPGSLLLLFLASHCCLGSARGLFLFGQPDFSYKRSNCKPIPVNLQLCHGIEYQNMRLPNLLGHETMKEVLEQAGAWIPLVMKQCHPDTKKFLCSLFAPVCLDDLDETIQPCHSLCVQVKDRCAPVMSAFGFPWPDMLECDRFPQDNDLCIPLASSDHLLPATEEAPKVCEACKNKNDDDNDIMETLCKNDFALKIKVKEITYINRDTKIILETKSKTIYKLNGVSERDLKKSVLWLKDSLQCTCEEMNDINAPYLVMGQKQGGELVITSVKRWQKGQREFKRISRSIRKLQC 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 2 |
| Calculated molecular weight | 295 aa, 34 kDa |
| Observed molecular weight | 38 kDa |
| GenBank accession number | BC008666 |
| Gene Symbol | SFRP2 |
| Gene ID (NCBI) | 6423 |
| RRID | AB_2881709 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96HF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| IHC protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| WB protocol for SFRP2 antibody 66328-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Exp Cell Res miR-629-5p promotes growth and metastasis of hepatocellular carcinoma by activating β-catenin. | ||
Front Immunol Integrated single-cell RNA sequencing reveals the tumor heterogeneity and microenvironment landscape during liver metastasis in adenocarcinoma of esophagogastric junction | ||
Nat Commun Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring
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