SFRP1 Polyclonal antibody
SFRP1 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 26460-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | A549 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue, SH-SY5Y cells, U2OS cells, mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SFRP1 Ready-To-Use IHC Kit. SFRP1 primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
The immunogen of 26460-1-AP is SFRP1 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SFRP1 fusion protein Ag24844 相同性解析による交差性が予測される生物種 |
| Full Name | secreted frizzled-related protein 1 |
| Calculated molecular weight | 314 aa, 35 kDa |
| Observed molecular weight | 30-35 kDa |
| GenBank accession number | BC036503 |
| Gene symbol | SFRP1 |
| Gene ID (NCBI) | 6422 |
| RRID | AB_2880522 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| IHC protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| IF protocol for SFRP1 antibody 26460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Exosome-mediated Hic-5 regulates proliferation and apoptosis of osteosarcoma via Wnt/β-catenin signal pathway. | ||
Cells Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Hua (Verified Customer) (03-14-2023) | There are doubts about if this antibody is working in WB. There are some non-specific bands and the supposed band is very weak.
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